Antioxidant Capacity Assays Services
Excess ROS must be rapidly removed from cells through a variety of antioxidant defense mechanisms. Cellular antioxidant enzymes and other redox molecules are used to balance reactive oxygen species produced in cells.
Creative Bioarray offers FRAP, TAC, ORAC, HORAC assay services. This service helps customers to measure the ability of the provided samples to neutralize specific types of free radicals. The types of samples we can accept include, biological fluids, cells and extracts.
Antioxidant Capacity Assays Services at Creative Bioarray
The Ferric Reducing Antioxidant Power (FRAP) Assay Service
Figure 1. Reaction scheme involved in FRAP assay. (Santos & Silva, 2020)
| Principle |
Sample form |
Applications |
| Measures antioxidant potential in samples through the reduction of ferric iron (Fe3+) to ferrous iron (Fe2+). |
Serum, plasma, lysates, biological fluids |
This method can also be used to assay the antioxidant capacity of purified food or drug extracts if required. |
The Total Antioxidant Capacity (TAC) Assay Service
| Principle |
Sample form |
Applications |
| Reduction of copper(II) to copper(I) |
Plasma, serum, urine, cell lysates, tissue homogenates and food extracts. |
Works with a wide variety of antioxidants. |
The Oxygen Radical Antioxidant Capacity (ORAC) Assay Service
Measures the ability to scavenge oxygen radicals. Suitable for use with plasma, cell fractions, tissue lysates, solid and aqueous nutrition samples.
The Hydroxyl Radical Antioxidant Capacity (HORAC) Assay Service
Measures the capacity to reduce hydroxyl radicals. Suitable for use with plasma, cell fractions, tissue lysates, solid and aqueous nutrition samples
Trolox Equivalent Antioxidant Capacity (TEAC) Assay
Figure 2. TEAC measuring principle and results expression. (Litescu et al., 2014,)
Suitable for use with plasma, serum, urine, cell lysates, tissue homogenates and food extracts.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you at the first time.
References
- Santos, C. M., & Silva, A. M. (2020). The antioxidant activity of Prenylflavonoids. Molecules, 25(3), 696.
- Litescu, S. C., Eremia, S. A., Tache, A., Vasilescu, I., & Radu, G. (2014). The use of oxygen radical absorbance capacity (ORAC) and Trolox equivalent antioxidant capacity (TEAC) assays in the assessment of beverages’ antioxidant properties. Processing and Impact on Antioxidants in Beverages, 245-251.
All services and products are for scientific use only, not for medical use！
