Cardiomyocyte Antioxidant Evaluation Service
The relationship between free radicals and diseases has become a hot topic in the field of modern medicine and life sciences. Many diseases are related to cellular oxidative stress caused by free radicals. Antioxidants can reduce or eliminate free radicals and prevent or reduce the damage caused by free radical oxidative stress. A large number of anti-free radical drugs or antioxidants have been developed, and even free radicals have been used as auxiliary indicators for disease diagnosis.
Creative Bioarray provides antioxidant rescue assessment services. Based on the established myocardial oxidative stress model, we can provide anti-free radical drug / antioxidant administration experiments, pharmacy pharmacology, efficacy evaluation, and pharmacokinetic analysis at the same time. The purpose of this service is to promote the screening and development of antioxidants and anti-free radicals in the circulatory system.
Service Content
As an expert in the field of cell stress, we can provide customers with one-stop services. At the same time, our experimental scheme is very flexible. Customers can choose different schemes according to their own needs.
Selection of cell lines
A stress model was established based on selected cardiac myocytes. We prefer the H9c2 cell line, which is a commonly used and readily available cell line. If you need to use other cell lines, you may need to provide them or authorize us to purchase them.
In addition, our commonly used stressors include sodium arsenite, a high glucose environment, and hypoxia/reoxygenation. The modeling method in a high sugar environment is more suitable.
Selection of induction method
Unless otherwise specified, we usually use H2O2 as a stressor, which is a cheap and commonly used modeling method with high modeling efficiency. The myocardial oxidative stress model established by H2O2 can meet most research purposes.
For some specific research purposes, we will also actively recommend more appropriate induction schemes for customers. For example, in our research on the relationship between diabetes and the myocardial system, we will use a high glucose environment to induce cellular oxidative stress. We will also stimulate the oxidative stress of myocardial cells in ischemic heart disease through hypoxia/reoxygenation.
Scheme 1- Model Establishment + Antioxidant Screening Service
We usually use hydrogen peroxide, or other substances as a source of oxidative stress. According to customer requirements, provide anti-oxygen free radical drug / antioxidant administration experiment, pharmaceutical pharmacology, efficacy evaluation, and pharmacokinetic analysis.
Scheme 2 - Anti-Oxygen Free Radical Drug / Antioxidant Screening Service
The customer provides established stress cell lines or oxidatively damaged tissues. After cell or tissue culture, we provide anti oxygen free radical drug / antioxidant administration experiment, pharmaceutical pharmacology, efficacy evaluation, and pharmacokinetic analysis. We can screen and evaluate the candidate drugs you provide, and also evaluate the antioxidant drugs you select.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
