Cell Oxidative Stress Assay Service Selection Guide
Oxidative stress refers to the increase of free radicals or the weakening of antioxidant protection ability under the stimulation of harmful factors in vivo and in vitro, resulting in the imbalance of oxidative system and antioxidant system. Here is a general guide to the oxidative stress assay service selection.
Q1: With so many markers of oxidative stress, what methods will Creative Bioarray use to provide customers with samples for oxidative stress detection?
A1: Oxidative stress can be tested directly by measuring the presence of various reactive oxygen species, or indirectly by measuring the damage to one or more biomolecules (DNA, RNA, protein or lipid). Indirect methods are more common due to the stability of most oxidative damage markers and the relative instability of many ROS.
Many customers require that oxidative stress be confirmed by testing a variety of oxidative stress markers. This can be done by testing multiple markers on a biomolecule (e.g. different markers of protein damage) or by testing one marker on different molecules (e.g. protein, lipid and DNA).
Here's the best way to start your oxidative stress study:
- Protein carbonyl ELISA
- 8-OHdG ELISA
- TBARS detection
Q2: Are reactive oxygen species formed outside oxidative stress?
A2: As part of normal metabolism, ROS is produced outside oxidative stress. These detection methods do not distinguish the source of ROS. However, ROS / damage levels are expected to increase when the sample is under pressure. Most oxidative stress studies measure relative changes in ROS production rather than absolute quantification.
Q3: What are the advantages or disadvantages of directly measuring ROS compared with damage markers on protein and DNA?
A3: Although direct measurement of ROS is ideal, it is difficult to detect ROS directly due to their transient characteristics. On the contrary, many researchers prefer to measure damage markers because they are often more stable and therefore easier and reliable to measure.
Q4: What species does Creative Bioarray's oxidative stress testing service apply to?
A4: Almost all of our testing services are species independent, which means they are applicable to samples of any species. This is because the detection is dedicated to measuring damage markers, not biomolecules themselves. For example, specific proteins may vary from species to species, but markers of protein damage, such as protein carbonyl and 3-nitrotyrosine, are always the same.
Q5: How to prevent further oxidation of samples during storage?
A5: In order to prevent further oxidation during storage, 0.005% BHT can be added to the sample. A methanol solution of 5% butylated hydroxytoluene can be prepared as a stock solution (1000x).
Q6: What is the most reliable method to detect lipid peroxidation in vitro?
A6: We provide a variety of detection methods for the following lipid peroxidation markers: 4-HNE, 8-Isoprostaglandin, MDA and oxidized LDL.
In addition to testing services, we also provide ELISA kits for HNE, isoprostaglandins, MDA and oxidized LDL markers. Our HNE adduct ELISA and MDA ELISA are similar kits, but the MDA adduct is not very stable, and the samples should be stored at - 80°C for no more than one month.
All services and products are for scientific use only, not for medical use！
