Characterization of Natural Lipid Antioxidant Activity in Food and Plants
The determination and characterization of antioxidant capacity of antioxidants is an important part of the field of cellular stress. Lipid oxidation is one of the main causes of food spoilage, and antioxidants can extend the shelf life of foods containing fats or oils. As a leader in the field of oxidative stress, Creative Bioarray offers solutions for the determination and characterization of antioxidant components resistant not only in organisms, but also in food products. We can screen and characterize active antioxidant substances from vegetables, fruits, herbs and other plants for our customers.
Phase 1: Antioxidant Screening of Plant-Derived Foods
Antioxidants and their oxidation products have no toxic side effects on human body
Stable during transportation, storage and food preparation
No adverse effects on the nutritional value and flavor of the food
Ensure effectiveness at low concentrations
Screening of resource plants. Extraction of resource plants using a variety of different treatments, and qualitative and quantitative analysis of the extracts obtained, and finally screening by determining whether the plant has a substance with known free radical scavenging ability and the level of content of that substance.
Screening of antioxidant capacity of plant-derived food antioxidants. One is to reflect the antioxidant capacity of the substance to be tested by determining the ability of the sample to inhibit the oxidation reaction of lipids in the test system under specific environmental conditions; the other is to reflect the antioxidant capacity of the sample by its ability to scavenge artificially generated free radicals.
Phase 2: Characterization of Natural Antioxidant Activity
- Determination of the ability of the sample to inhibit the oxidation reaction of lipid substances in the test system
By adding the substance under test, the length of time or reduction in the amount of reactants, intermediates and end products present during oxidation indicates that the substance under test has antioxidant activity. Our methods for measuring changes in the amount of reactants, intermediates or end-products during lipid oxidation include
| Chemical method |
Peroxide value, iodine value, acid value, TBARS method, carbonyl compounds, Kress test and anisidine value. |
| Physical method |
Measurement of viscosity, color, conjugated diene content, refractive index, dielectric constant
Infrared spectroscopy, gas chromatography, liquid chromatography, combined gas chromatography and mass spectrometry
Measurement of the ratio of unsaturated fatty acids to saturated fatty acids, content of polymers, content of polar lipids
Oxygen pressure method to measure pressure changes due to the reduction of oxygen in the system, oxygen electrode method to measure oxygen consumption, measurement of the reduction of unsaturated fatty acids in the substrate and weight gain of the substrate after oxidation |
- Determination of the ability to scavenge artificially generated free radicals
The level of antioxidant activity was determined based on the strength of the ability of the test substance to scavenge free radicals.
The artificially generated free radicals we commonly use are ABTS+, DMPD+, DPPH∙, Fremy radicals, and galvinoxyl radical amount.
- Determination of antioxidant reducing ability
Metal ions are catalysts for oxidation, and antioxidants can chelate metals and decompose peroxides, thus suspending free radical-initiated oxidation before it occurs.
Our common methods include FRAP method, CUPRAC method, FC method and CV method.
- Determination of antioxidant inhibition of oxidative degradation of substrates by free radicals
In these methods, both substrates and free radicals are present in the test system, and the substrates react with exogenous free radicals to form fluorescent or colored characteristic substances, and the antioxidant activity can be measured by fluorescence or absorbance reduction.
Our commonly used methods include ORAC method, TRAP method, DCFH-DA method, chemiluminescence method, B-carotene bleaching method, saffron bleaching method, KI method, and TOSC method.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
