Characterization of Pathological Sample Damage Fingerprint

Oxidative stress (OS) is associated with the development of human diseases. Early identification of OS-associated diseases is critical to control their progression and treatment. Efforts have been made to identify reliable endogenous markers that correlate with disease progression in organs that receive OS.

The identification of reliable biomarkers, such as modified endogenous compounds formed in cells and organs as a result of OS, is critical for characterizing OS and predicting the early development of pathological conditions.

As a leader in the field of cellular stress, Creative Bioarray can provide our customers with solutions for the characterization of oxidative stress in pathological samples (body fluids, tissues, and cells) using designed exogenous reporters. By utilizing these markers, it is possible to correlate the damage fingerprint of the marker to specific pathological conditions.

Technical Description

The exogenous reporters we use include the design and synthesis of sensitive molecules that consist of various endogenous subunits covalently linked together to form new probes. The designed marker consists of an amino acid tyrosine (T) linked to linoleic acid (L), forming an amide bond. Other endogenous units that can be added to LT to form esters with tyrosine carboxyl groups may include cholesterol, yielding linoleoyl tyrosine cholesteryl ester (LTC), or 2'-deoxyguanosine, yielding linoleoyl tyrosine 2'-deoxyguanosine ester (LTG).

Applicable Sample Range

Body fluids: urine, serum, whole blood, saliva
Tissues: brain or muscle homogenates
Cells: macrophages, endothelial cells, astrocytes, neurons

Our Approaches

Step 1: Test for these types of exogenous markers in biofluid fluids, in cell systems, and in vivo by injection (intramuscular or intravenous) into specific animals (which are known to develop OS-related diseases)

Step 2: Incubation of fluid, tissue, or cultured cells with synthetic redox reporter molecules

Step 3: Extraction of the probe and its oxidation products (fluid or whole cells)

Step 4: Analysis of probes (LT and LTG) and their oxidation products (Ox-LT and Ox-LTG) by LC/MS methods

Step 5: After incubation and extraction, each dried probe sample is dissolved in a mixture of methanol and acetonitrile in an eluent of the above gradient and monitored by an MS detector. each compound in the LC chromatogram is further decomposed in the mass spectrogram to reveal its specific molecular ion and its characteristic decomposition.

Our Solutions are Expected to Help Our Customers to Reveal

Possible oxidative modifications of polyunsaturated fatty acids, amino acids, sterols,, and nucleic acids
Types of products formed during OS
Relative oxidative sensitivity of the elements used to construct the probes under defined OS conditions
Effectiveness of specific interventions (e.g., supplemental antioxidants)

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All services and products are for scientific use only, not for medical use！