Chicken Embryo Oxidative Stress Modeling Service
At present, the methods of studying oxidative stress and antioxidation mainly include an animal model method and the cell model method. Although the establishment of a cell model is rapid, only a single cell model in Vitro cannot reflect the complex physiological processes and mechanisms in Vivo.
As a classical developmental model, the chicken embryo is widely used in various fields of biology. Embryos are sensitive to external stimuli during development, and oxidative stress is one of the mechanisms leading to early embryonic development damage. Previous studies have used various stressors to establish different chicken embryo, oxidative stress models. As a leader in the field of cell stress, Creative Bioarray provides our customers with chicken embryo oxidative stress modeling services. The model can be used to explore the adverse effects of oxidative stress on embryonic development, study the mechanism of disease, and screen and evaluate the activity of drugs.
Model Features
The chicken embryo experiment has the advantages of a clear development process, easy operation, and low price. As a classical embryonic developmental model, it is widely used in biology, oncology, virology, pharmacology, and other related fields.
The establishment of the embryonic oxidative stress model is of great significance to reveal the mechanism of embryonic stress load and study the role of protective substances.
Available Modeling Methods at Creative Bioarray
We used AAPH chicken embryos to establish an oxidative stress model to reveal the mechanism of oxidative stress injury and screen antioxidants. AAPH is a water-soluble azo compound, which can cause typical lipid peroxidation by thermal decomposition at physiological temperature. Because the rate of free radicals produced by AAPH is easy to control and measure, it has a stable thermal degradation constant, and there is no biotransformation in the decomposition process. It is a widely used product of thermal degradation free radicals. Therefore, initiating free radicals with AAPH provides a good method to induce membrane damage.
After AAPH treatment, the allantoic membrane vessels of chick embryos were seriously damaged, the total antioxidant index of heart tissue was significantly reduced, and the content of MDA was significantly increased, suggesting that AAPH may cause oxidative stress damage in embryonic heart tissue.
Model Applications
- Application of chicken embryo oxidative stress model in disease research
Many clinical events, such as fetal dysplasia or preterm birth, are related to oxidative stress load, and an appropriate amount of reactive oxygen free radicals. During the early development of chicken embryos, the expression and function of development-related genes are very similar to that of human embryos, so it is very suitable to study the related diseases of embryonic development.
- Application of chicken embryo cell oxidative stress model in drug activity evaluation
Chicken embryo fibroblasts and skeletal cells are often used in the study of oxidative stress. They are stimulated by various inducers in Vitro. After induction, they can cause changes in the main indicators of oxidative stress, such as the increase of lipid peroxidation level, the decrease of antioxidant enzyme activity, the enhancement of apoptotic protease activity, and finally lead to the increase in cell mortality.
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