Chondrocyte Biological Oxidative Stress Modeling Service
Chondrocytes are the only c ell type in mature cartilage and are responsible for repairing damaged cartilage tissue. Because articular cartilage is vascularized, regeneration after injury or degenerative changes is very limited. Chondrocyte apoptosis will change the synthesis of cartilage matrix and lead to cartilage degradation and destruction. Accumulated evidence shows that oxidative stress is related to chondrocyte apoptosis.
Creative Bioarray uses Interleukin-1 β and tumor necrosis factor induce oxidative stress and endoplasmic reticulum stress in human chondrocytes. This is a modeling method using biological stressors. If you need, we can use chemical stressors to provide you with chondrocyte oxidative stress model building services.
Technology Principle
Endoplasmic reticulum stress (ERS) is an important way for cells to respond to environmental changes, initiate self-response, self-repair and regulate apoptosis. It is closely related to the occurrence of many diseases, including osteoarthritis (OA). Oxidative stress can interfere with cartilage homeostasis and promote catabolism by inducing apoptosis. Excess reactive oxygen species can lead to mitochondrial dysfunction, which is the main cause of cell injury and death under various pathological conditions.
Interleukin-1 β（1L-1β）, Tumor necrosis factor (TNF-α) changes in the expression and regulation of inflammatory response related factors, such as, will activate related signal pathways, make the metabolism of joint cells lose balance, and lead to cell aging and apoptosis, leading to the further aggravation of joint inflammation.
What We Do
Chondrocyte culture and modeling
Isolation and culture of chondrocytes: the isolation and culture of osteoarticular chondrocytes were carried out according to the sequential digestion of type II collagenase combined with trypsin method. After subculture, use different concentrations of 1L-1β and TNF-α. The expression level of related factors was detected after 24 hours, and the apoptosis level was detected after 72 hours.
Modeling quality evaluation system
RT-PCR was used to detect the mRNA levels of inflammatory factor 1L-1β and TNF-α, as well as ERS related factors GRP78 and CHOP in cartilage.
The expressions of GRP78, chop, ATF4 and Caspase-3 in cultured chondrocytes were detected by Western blot.
Apoptosis was detected by TUNEL method.
Model Application
We can also adjust the modeling process according to the specific needs of customers, provide administration experiment, pharmacology, efficacy evaluation and pharmacokinetic analysis, and assist customers to study chondrocyte stress and / or pathological significance, which has important theoretical significance for clarifying chondrocyte related diseases.
The model can also be used as an endoplasmic reticulum oxidative stress model of chondrocytes.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you at the first time.
All services and products are for scientific use only, not for medical use！
