Chondrocyte Chemical Oxidative Stress
Modeling Service
Chondrocytes are the only cell type in mature cartilage and are responsible for repairing damaged cartilage tissue. Because articular cartilage is vascularized, regeneration after injury or degenerative changes is very limited. Chondrocyte apoptosis will change the synthesis of cartilage matrix and lead to cartilage degradation and destruction. Accumulated evidence shows that oxidative stress is related to chondrocyte apoptosis.
Creative Bioarray uses H2O2 oxidative stress and endoplasmic reticulum stress in rat and human chondrocytes. This is a modeling method using chemical stressors. If you need, we can use biological stressors to provide you with chondrocyte oxidative stress model building services.
Service content
Culture of various cells and establishment of cell models (drug treatment, transfection, changes in various environmental conditions, etc.), cell line culture and cryopreservation.
In short, chondrocytes were isolated from the articular cartilage of three-week-old male rats. The cartilage was removed from the animal and then euthanized by excessive anesthesia. The cartilage was sliced, washed with sterile phosphate buffered saline (PBS), and then digested with type II collagenase in DMEM overnight in an incubator at 37°C. The digested cartilage was collected and centrifuged. The particles were resuspended in DMEM and filtered through a cell filter. Chondrocytes divided into 2-3 generations and were used in subsequent experiments.
Cell Viability Assay
In order to evaluate the time response relationship of H2O2 in the experiment, cell viability was evaluated by CCK-8 assay. Chondrocytes were plated at the density of 96 well plates, and cells / wells adhered overnight and treated with H2O2 for 0, 0.5, 1, 2, 4, 12 and 24 hours. After the incubation time, add CCK-8 solution and further incubated in 37°C and 5% CO2 for 1 hour. The absorbance of 450 nm-650 nm was measured by microplate reader.
Other Modeling Indicators
- Apoptosis assay
- Measurement of ROS production in chondrocytes
- MDA production and CAT activity were measured
- SOD-2 activity detection
- Measurement of caspase-9 and caspase-3 activities
The indicators above can be analyzed to make evaluation of the model.
Service Description
We provide conventional chondrocyte culture medium. If special culture medium is required, corresponding culture medium shall be provided.
Basic Service Process
- Cell resuscitation and culture
- Establishment of cell model
- Relevant function analysis
- Experimental report
Service Report
The experimental report includes detailed experimental report, original experimental data and data result analysis.
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All services and products are for scientific use only, not for medical use！
