Comprehensive In Vitro Oxidative Stress Monitoring Solutions
Oxidative stress is a well-known phenomenon caused by a shift in the delicate balance between the generation of free radicals in cells and their ability to be scavenged. Reactive oxygen species (ROS) are mainly composed of superoxide radicals, hydroxyl radicals, etc. Usually, in vitro systems are the first line of experimental protocol for any scientific hypothesis, therefore cell culture and simulated oxidative stress are usually followed by various assays to assess oxidative stress. As a leader in the cellular stress industry, Creative Bioarray offers a comprehensive solution for in vitro assays for oxidative stress determination.
Spectrometry-Based Biochemical Assay Solutions
Hydrogen Atom Transfer Methods
These solutions are used to measure antioxidant capacity or to scavenge ROS by loss of hydrogen ions.
- Oxygen radical absorbing capacity (ORAC) assay
- Lipid peroxidation inhibition capacity (LPIC) assay
- Total radical-trapping antioxidant parameter (TRAP) assay
- ABTS assay
Electron Transfer Methods (ETMs)
- TEAC assay
- FRAP assay
- DPPH assay
- Total phenols estimation by Folin-Ciocalteu
Other Related Spectrometric Methods
- Dye-based fluorometric assay in cell lysate
The degree of fluorescence in the sample is proportional to the amount of ROS; however, these methods do not allow for absolute quantification, only relative quantification.
- TBARS assay
Flow Cytometry-Based Assay Solutions
The advantage of flow cytometry-based assays is that they represent a semi-quantitative assessment and also distinguish between cells that show different levels of ROS. We have a variety of commercially available fluorescent probes that show binding to specific types of reactive oxygen species (ROS).
- Hydrogen peroxide probes
- Hydroxyl radical probes
- Hypochlorous acid probes
- Peroxynitrite anion probes
- Superoxide anion probes
Electron Spin Resonance (ESR)-Based Assays
Electron spin resonance or ESR spectroscopy is the most advanced technology that has recently received attention. This technique is powerful enough to specifically detect free radicals involved in autoxidation and cross-oxidation processes.
Microscopy-Based Assays
Use fluorescence microscopy to directly observe the production and persistence of reactive oxygen species. This has two advantages. Firstly, it can be used to confirm and correlate flow cytometry results; secondly, it can help the user to exclude artifacts. In addition, many dyes (fluorescent probes) can be used to determine the secondary effects of oxidative stress, such as changes in mitochondrial membrane potential or direct measurement of mitochondrial superoxide (MitoSox).
Cell Model for In Vitro Oxidative Stress Assay
H9c2 was used as the preferred model system. H9c2 is basically an incompletely differentiated cell, derived from the heart, hence the name cardiomyocytes. These cells perform well in artificial stimulation and different types of oxidative stress simulations. Various studies in many other research laboratories have demonstrated that H9c2 can be used in studies related to oxidative stress.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
