Detection of Liver Disease Progression by Oxidative Stress Status Assessment
Redox status influences the course of inflammatory, metabolic and proliferative liver disease. Oxidative stress is thought to play a critical and ongoing role in the pathological progression of early steatosis to severe hepatitis, fibrosis, cirrhosis, and hepatocellular carcinoma. As a leader in the cellular stress industry, Creative Bioarray offers a solution for detecting liver disease progression through oxidative stress status assessment. This is a non-invasive test that avoids problems caused by liver biopsy (e.g. bleeding or infectious complications, test attrition, sampling errors, and differences in diagnostic classification between pathologists). The solution is currently suitable for
- Monitoring the protective effects of multiple antioxidants in mice for the prevention of liver disease in humans
- Monitoring the dynamics of redox metabolism in mice in the early stages of liver disease
- Note that our solution is intended for scientific research only and not for medical use
Solution for Detecting Liver Disease Progression by Oxidative Stress State Assessment
In vivo dynamic nuclear polarization magnetic resonance imaging (DNP-MRI) allows for monitoring the anatomical distribution of free radical species. The strategy we used was in vivo redox metabolism imaging by DNP-MRI using carbamoyl-PROXY (CMP) as a molecular imaging probe.
Redox Status Monitoring Service for Mice with Liver Disease
- Redox imaging of DNP-MRI in liver disease mice in vivo
Model Establishment. We have established a mature animal modeling platform. We can build mouse models of liver disease (e.g., nonalcoholic fatty liver disease through a methionine-choline deficient diet) in a targeted manner according to customer needs.
In vivo imaging. Enhanced DNP-MRI is performed every few seconds after intravenous injection of CmP into mice. the distribution of enhanced signal areas can be observed throughout the liver minutes after the injection is completed.
- Quantification of oxidized and total CmP in the liver
A solution containing the probe is injected intravenously into the mice for several weeks. Liver tissues from mice were collected and the concentration of nitrilyl radicals in each sample was measured by X-band EPR. Total carbamoyl-PROXYL was measured after the addition of ferricyanide.
- Assessment of CmP redox metabolism in liver tissues using X-band EPR
- Isolation of mitochondria and detection of CmP redox metabolism therein
The Solution is Expected to Solve
- The diagnosis of liver disease requires an invasive liver biopsy, which can lead to bleeding or infectious complications, test attrition, and sampling errors. To avoid these problems you may need to assess the progression of liver disease in study subjects with a non-invasive type of test.
- When you need to visualize disease progression as a redox map, allowing liver disease to be quantitatively assessed by in vivo imaging at an early stage.
- When you need to perform redox metabolic imaging of a mitochondrial model of the liver.
- When you need to dynamically monitor the progression of liver disease, this non-invasive assay detects excessive oxidative stress earlier than by immunohistochemical analysis of 8-OHdG and 4-HNE.
- This solution can be used as an assessment of mitochondrial redox status in the context of liver disease to evaluate the extent of liver injury and ultimately the response to drug therapy.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
