Detection of Oxidative Stress-Related MicroRNAs for Disease Diagnosis
MicroRNAs (miRNAs) are short non-coding RNAs of 18-25 nucleotides that are key to many new discoveries related to biological phenomena and pathology, and are specific targets for diagnostic and prognostic applications.
Oxidative stress stimulates the production of several miRNAs, which are termed oxidative stress-responsive miRNAs. they further play a role in linking dysregulated antioxidant defense systems to an unbalanced redox state. These miRNAs have been identified as potential targets for the diagnosis and prognosis of cardiovascular, liver and neurodegenerative diseases, diabetes, sepsis and different types of cancer.
Creative Bioarray can provide clients with oxidative stress-related microRNA assays at the extracellular and/or intracellular levels and diagnostic and prognostic assessment of diseases in target experimental animals by analyzing the expression levels of the target miRNAs. Research on the functional and regulatory aspects of oxidative stress-related miRNAs will provide new directions for the discovery of new diagnostic and prognostic biomarkers.
Please note that our solutions are intended for scientific research only and are not intended for medical use.
miRNA Fluorescence Quantitative Testing Services
Two assays are available for miRNA fluorescence quantification services
- Probe method
- miRNA fluorescence quantification service dye embedding method
miRNA fluorescence quantification service
- Primer design
- RNA extraction
- RNA reverse transcription for cDNA synthesis
- Real-time PCR reactions
- Data analysis
miRNA fluorescence quantitative assay service results
Experimental reports, detailed experimental methods, real-time fluorescence quantitative PCR experimental results, graphs and related analytical data are provided.
Detectable Oxidative Stress-Related MicroRNAs for Disease Diagnosis
| Diseases |
Oxidative Stress-Related MicroRNAs |
| Cardiovascular Diseases |
miR-200 family (miR-141 and miR-200c), miR-34a, miR-200a, miR-21 |
| Liver Injury |
Association between oxidative stress and miR-122 in tuberculosis drug-liver injury (ADLI) |
| Neurodegenerative diseases |
miR-153 |
| Sepsis |
miR-25, biomarkers that play an important role for the timely diagnosis and assessment of sepsis status |
| Diabetes mellitus |
miR-1, miR-133a, miR-133b and miR-499 |
| Ovarian cancer |
miR-141, miR-200ami, miR-200 family |
Sample Provision
RNA samples. Take appropriate amount of fresh tissues, homogenize and extract total RNA, and provide RNA quality test chart.
Tissue samples. Take appropriate amount (50-100mg) of tissue preserved in liquid nitrogen.
Blood samples. Add coagulant and centrifuge, take serum, plasma or whole blood for analysis.
Cell samples. Take 107 cells, aspirate the culture fluid and then extract RNA with TRIZOL reagent.
Technical Features
- High specificity, allowing quantification of only mature microRNAs, effectively distinguishing mature microRNA molecules from precursor molecules and other homologs of mature microRNAs.
- Fast, simple and time-saving, the whole operation takes only two steps, less than 3 hours, to obtain high quality data.
- High sensitivity and low sample consumption, requiring only 1-10ng of total RNA or 50pg of microRNA.
- Ultra-wide linear range spanning 7 orders of magnitude.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
