1-631-626-9181
Development and Evaluation of Amino Acids & Peptides-based Antioxidants

The mitochondrial respiratory chain on the inner mitochondrial membrane (IMM) is the main intracellular source of reactive oxygen species (ROS). Mitochondria are continuously exposed to ROS and accumulate oxidative damage faster than other parts of the cell. Many studies have linked ROS-induced mitochondrial dysfunction to necrotic and apoptotic cell death.

Molecular structure of the two mito-targeted antioxidants and their situation into the inner mitochondrial membrane and electron transport chain.Fig. 1 Molecular structure of the two mito-targeted antioxidants and their situation into the inner mitochondrial membrane and electron transport chain. (Cantarero et al., 2020)

The most effective way to inhibit ROS-induced cytochrome C release and cell death is to prevent lipid peroxidation in the IMM.

However, there is no available antioxidant that specifically targets mitochondria, let alone IMM. in addition, most antioxidants have poor cellular permeability and require concentrations greater than 100 μm to prevent oxidative cell death.

As a leader in the field of cellular stress, Creative Bioarray can help our customers develop a range of peptide antioxidants that are taken up by mitochondria and concentrated in the IMM.

Strategies for the Development of Antioxidants Targeting Mitochondria

We can help our customers develop a new class of small cell permeable peptide antioxidants targeting mitochondria. The basic structural motif of these peptides consists of alternating aromatic residues and basic amino acids. These peptides contain a sequence of amino acids that are free to penetrate the cell despite carrying a net 3+ charge at physiological pH, and they are highly concentrated in the mitochondria.

Processes and Strategies for the Synthesis and Evaluation of Antioxidants Targeting Mitochondria

  • Synthesis of Targeted Antioxidants

We will customize the appropriate synthesis according to the customer's objectives.

  • In vitro antioxidant performance measurement

The ability of targeted antioxidants to scavenge H2O2 in vitro is measured using luminol chemiluminescence assay. The antioxidant properties are further validated by inhibition of fatty acid peroxidation and low-density lipoprotein (LDL) oxidation.

  • Mitochondrial uptake and localization monitoring

To verify the uptake of targeted antioxidants by isolated mitochondria. Three cycles of freeze-thaw treatment were used to separate the inner and outer membranes to determine the localization of the peptide within the mitochondria.

  • Test whether peptide antioxidants reduce oxidant-induced intracellular ROS and cell death
  • Other Evaluation Items

Our evaluation of candidate peptide antioxidants includes, but is not limited to, cellular uptake and intracellular localization of peptide antioxidants, intracellular ROS and cell viability, intracellular mitochondrial potential, mitochondrial H2O2 production, mitochondrial oxygen consumption, mitochondrial membrane potential, mitochondrial swelling assay, mitochondrial cytochrome C release, animal model based studies.

How We Guarantee the Quality of Our Services

  • We have a professional research team that can design mitochondria-targeted antioxidant delivery systems according to customer requirements
  • We have a proven biological and chemical synthesis platform to handle antioxidant synthesis tasks
  • We have rich experience in providing complete targeted antioxidant evaluation solutions
  • We have a proven animal testing platform to simulate multiple diseases for in vivo targeted antioxidant evaluation

Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.

Reference

  1. Cantarero, A., Mateo, R., Camarero, P., Alonso, D., Fernandez‐Eslava, B., & Alonso‐Alvarez, C. (2020). Testing the shared‐pathway hypothesis in the carotenoid‐based coloration of red crossbills. Evolution, 74(10), 2348-2364.
All services and products are for scientific use only, not for medical use!

Solutions

Online Inquiry

About Us

Creative Bioarray is a leading customer-focused biotechnology company. We are dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development.

Contact Us

  • SUITE 115, 17 Ramsey Road, Shirley, NY 11967, USA
  • 1-631-626-9181
Copyright © 2025 Creative Bioarray. All Rights Reserved.

We use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. Read our Privacy Policy

Accept Cookies
x