Development and Evaluation of Lipophilic Cations-based Antioxidants
Reactive oxygen species (ROS) play an important role in the pathogenesis of a variety of diseases or pathological processes, including various forms of cancer, type 2 diabetes, atherosclerosis, chronic inflammation, ischemia/reperfusion injury, sepsis, and some neurodegenerative diseases. Mitochondria are a major intracellular source of ROS under pathological conditions and there is increasing interest in the concept of developing mitochondria-targeted antioxidants as therapeutic agents. However many of the most extensively studied compounds fail to localize within the mitochondria or even enter the intracellular environment.
The most common approach to targeting compounds to mitochondria is to exploit the potential gradient of the inner mitochondrial membrane, which causes the matrix side of the membrane to be negatively charged. Due to the electrolysis of the inner membrane, lipophilic cations tend to accumulate within the mitochondrial matrix. As a leader in the field of cellular stress, Creative Bioarray can provide our customers with solutions for the development and evaluation of lipophilic cation-based antioxidants.
Strategies for the Development of Mitochondria - Targeting Antioxidants
Oxidants such as vitamin E, panthenol, and N-acetylcysteine have been shown to reduce mitochondrial oxidative damage, but their effectiveness is limited by the fact that these compounds do not accumulate within the mitochondria.
To develop antioxidants that target mitochondria, we attached antioxidants to lipophilic cations. The lipophilic cations should deliver their attached antioxidants to the mitochondria, thereby preventing mitochondrial oxidative damage.
Antioxidants attached to lipophilic cations accumulate rapidly through isolated mitochondria and intracellular mitochondria. Importantly, due to this selective accumulation, the targeted antioxidants protect mitochondrial function from oxidative damage more effectively than by themselves.
Synthesis and Evaluation of Antioxidants Targeting Mitochondria
- Synthesis of targeted antioxidants
We customize the appropriate synthesis according to the client's target antioxidants (vitamin E, panthenol and N-acetylcysteine, etc.). Accumulation of antioxidants by isolated mitochondria.
- Testing the protective effect of antioxidants against oxidative damage in mitochondria
Incubate mitochondria with targeted antioxidants to verify their role in preventing oxidative damage and reducing lipid peroxidation (thiobarbituric acid reactive substances) and protein damage (protein carbonyl compounds).
- Confirm the antioxidant effect of target antioxidants by blocking them with uncoupling agents.
- Determine whether targeted antioxidants are taken up by mitochondria in human cells.
- Determine the cumulative efficiency of targeted antioxidants in mitochondria
- In vivo testing in whole animal disease models
How We Guarantee the Quality of Our Services
- We have a professional research team that can design mitochondria-targeted antioxidant delivery systems according to customer requirements
- We have a proven biological and chemical synthesis platform to handle antioxidant synthesis tasks
- We have rich experience in providing complete targeted antioxidant evaluation solutions
- We have a proven animal testing platform to simulate multiple diseases for in vivo targeted antioxidant evaluation
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
