Development and Evaluation of Nitroxide Radicals-based Antioxidants
ROS are implicated in the pathogenesis of a variety of diseases or pathological processes, including various forms of cancer, type 2 diabetes, atherosclerosis, chronic inflammation, ischemia/reperfusion injury, sepsis, and some neurodegenerative diseases. Mitochondria are an important source of reactive oxygen species and therefore the development of mitochondria-targeted antioxidants as therapeutic agents is a promising therapeutic direction.
As a leader in the field of cellular stress, Creative Bioarray can provide our customers with solutions to develop a range of electron and ROS scavengers targeting mitochondria.
Strategies for the Development of Antioxidants Targeting Mitochondria
We help our customers to develop a range of targeted mitochondrial electron and ROS scavengers. The targeted antioxidants will consist of a "payload" (molecular fraction with electron and ROS scavenging activity) and a "targeting fraction" (molecular fraction that promotes selective accumulation in the mitochondria). For the payload fraction, these molecules utilize stable nitrogen-oxygen radicals.
An important aspect of the design of these molecules is the use of targeting sequences that preferentially deliver scavengers to the mitochondrial membrane.
Processes and strategies for the Synthesis and Evaluation of Antioxidants Targeting Mitochondria
- Synthesis of Targeted Antioxidants
- In vitro antioxidant performance measurement
- Confirmation of the concentration of antioxidants in mitochondria
Due to the presence of unpaired electrons, nitrogen oxides display a unique characteristic triplet state signal when analyzed by electron paramagnetic resonance (EPR) spectroscopy. Therefore, we use EPR spectroscopy to monitor the cellular delivery and metabolic fate of targeted antioxidants.
- Animal model-based assessment strategy
Establishment of suitable animal clover for oxidative stress
Measurement of biochemical parameters
Measurement of peroxidase activity
Measurement of cellular damage
Measurement of cytotoxicity
Measurement of malondialdehyde levels
Histological evaluation
Characteristics of Targeted Antioxidants
By accepting an electron, the nitrogen-oxygen radical is converted to the corresponding hydroxylamine. Hydroxylamines, as effective reactive oxygen scavengers, are converted back to nitrogen oxides in this process. In addition, nitroxide radicals have SOD-mimetic activity, which helps to prevent the binding of O2- to nitric oxide and thus inhibits the highly toxic substance ONOO-. Thus, nitrogen oxides combine several important antioxidant and electron scavenging properties in a single functional part.
How We Guarantee the Quality of Our Services
- We have a professional research team that can design mitochondria-targeted antioxidant delivery systems according to customer requirements
- We have a proven biological and chemical synthesis platform to handle antioxidant synthesis tasks
- We have rich experience in providing complete targeted antioxidant evaluation solutions
- We have a proven animal testing platform to simulate multiple diseases for in vivo targeted antioxidant evaluation
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
