Development and Preclinical Evaluation of Liposome-based Antioxidants for Diabetes
Diabetes mellitus is one of the major global public health problems. A large body of recent evidence suggests that imbalance in cellular redox status leads to oxidative stress in pancreatic β-cells and promotes the development of diabetes and related complications. Dietary antioxidants, such as curcumin, have been shown to have therapeutic effects in diabetes. However, these antioxidants have poor solubility, permeability and stability in conventional delivery systems, ultimately leading to their poor oral bioavailability.
Solid lipid nanoparticles (SLN) are a novel drug delivery system being developed in recent years, which combines the advantages of drug-containing microemulsions and liposomes as drug carriers, allowing for improved bioavailability, good physiological compatibility, controlled drug release and good targeting.
As a specialist in cellular stress, Creative Bioarray uses solid natural lipids as carriers to encapsulate antioxidants promising for the treatment of diabetes in lipid-like nuclei. This strategy significantly improves the oral bioavailability of antioxidants and provides the basis for the development of antioxidant drugs for the treatment of diabetes.
Our liposome-based diabetic antioxidant development and preclinical evaluation solutions include, but are not limited to
- Manufacturing liposome-based diabetic antioxidant lipid particles by encapsulating the customer's desired antioxidant in a lipid-like core.
- Quality testing and characterization of antioxidant-loaded liposomes.
- Design of animal oral dosing assays to determine antioxidant efficacy and bioavailability of antioxidant-loaded liposomes.
How Liposome Improve the Bioavailability of Your Antioxidants
- Antioxidants are taken up directly by the gastrointestinal tract in the form of liposome encapsulation. The mechanisms involved in this uptake include particle diffusion through mucus and entry into the surface of intestinal epithelial cells, epithelial interactions and cellular transport, as well as cytostasis and systemic dissemination.
- Gastrointestinal absorption of low water soluble drugs can be enhanced by emulsification to increase surface area.
- By incorporating liposomes, antioxidants can be embedded in the phospholipid bilayer. This reduces their contact with bacteria and enzymatic degradation during absorption.
- The encapsulation also allows prolonged contact with the intestinal wall due to the adhesion properties exhibited by the liposomes to the epithelial mucosal surface of the small intestine.
Preparation and Characterization of Diabetes Antioxidants Encapsulated Liposome
Preparation of antioxidant solid lipid nanoparticles
The prescribed amount of antioxidant, glycerol monostearate and lecithin were dissolved in a certain amount of dichloromethane, and the solvent was recovered under reduced pressure to make the film-forming material form a uniform film on the wall of the flask. Another polysorbate solution was taken and placed in the film-forming flask, and the film was eluted by rotation, and the antioxidant-liposome suspension system was obtained by ultrasonic treatment, and stored at 4℃ for backup.
Determination of antioxidants in liposomal particles by UV spectrophotometry
Determination of drug loading and encapsulation rate
Antioxidant-SLN particle size and zeta potential investigation
Preclinical Experiments on the in vivo Bioavailability of Antioxidant-laden Liposome
Establish mouse models of diabetes or other related diseases. Grouped oral antioxidant/carrier-liposome dosing to assess
- Plasma pharmacokinetics of antioxidant agents
- Plasma antioxidant activity after antioxidant-SLN administration
- Antioxidant concentrations in the blood of diabetic patients
- Blood glucose and insulin levels after dosing
- Effect of antioxidant-SLN on oxidative stress and antioxidant system in animals
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
