DNA & RNA Oxidative Stress Detection
DNA is one of the important target molecules of free radicals, especially reactive oxygen species. The oxidative damage of DNA caused by free radicals can lead to many consequences such as strand breakage and base modification of DNA molecules, which is related to the development of many cancers including colon cancer, breast cancer and prostate cancer. Mutations in checkpoint kinases eventually lead to reduced DNA repair and increased cancer susceptibility.
Recently, oxidative RNA damage associated with a variety of neurological diseases has been described, including Alzheimer's disease, Parkinson's disease and Lewy body dementia. The standardization of DNA oxidative damage detection methods is very important for relevant research. Creative Bioarray provides a variety of DNA / RNA oxidative damage detection services to measure the level of oxidative stress of cells.
8-OHG RNA Damage Assay
Recently, oxidative RNA damage has been associated with a variety of neurological diseases, including Alzheimer's disease, Parkinson's disease, Lewy body dementia, prion disease and subacute sclerosing encephalitis.
We used ELISA to detect the most common marker of RNA damage: 8-hydroxyguanosine (8-OHG). This service is applicable to the detection of RNA damage in urine, serum, cerebrospinal fluid, cells or tissues.
8-OHdG DNA Damage Assay
Among many types of oxidative DNA damage, 8-hydroxydeoxyguanosine (8-OHdG) is a ubiquitous marker of oxidative stress. 8-OHdG is one of the by-products of DNA oxidative damage. It is physiologically formed and enhanced by chemical carcinogens. The testing service is applicable to urine, serum, cells and other samples.
AP Sites Quantitation
The service is suitable for cell or tissue testing. DNA damage can be characterized by the formation of purine free or pyrimidine free (AP or debase) sites. It is estimated that this spontaneous base loss in mammalian cells is between 50000 and 200000 sites per day. Unrepaired base free sites inhibit transcription and may have mutagenic effects.
Comet Assays and Slides
Comet assay is a well-known method for measuring cell DNA damage. Our comet assays provides a quick and convenient way to screen for general DNA damage, regardless of the source of the sample.
DNA Double-Strand Break Assay
Double strand break (DSB) in DNA is one of the most dangerous types of DNA damage in cells. One of the earliest cellular responses to double strand breaks is the phosphorylation of histone variant H2AX at the site of DNA damage. When DSB is induced in mammalian cells, ser139 is phosphorylated within seconds. Phosphorylation of this serine residue leads to chromatin condensation and appears to play a key role in the recruitment of repair or damage signaling factors to DNA damage sites. We can detect double strand breaks in just a few hours by immunofluorescence staining of phosphorylated histone H2AX.
Poly (ADP-Ribose) ELISA Assay
This service is applicable to cell or tissue samples and can detect par as low as 20 pm.
Poly ADP ribosylation (PAR) involves various biological functions, but one of the most studied functions is to produce par chain in response to DNA damage. Such par chains recruit repair proteins and chromatin remodeling agents to enhance the DNA repair process. We can quickly detect and quantify par levels in cell or tissue samples. The number of par in the unknown sample is determined by comparing its optical density with the optical density of the known standard curve.
UV Induced DNA Damage Assays
DNA may be damaged by ultraviolet light, resulting in the formation of two structures: cyclobutane pyrimidine dimer (CPD) and pyrimidine (6-4) pyrimidinone photoproduct (6-4pp). The result was mutations from C to T and CC to TT, the most common mutation in p53 found in human and mouse skin cancers.
We provide assays to quantify cpd or 6-4pp in three forms:
- ELISA detection for intact cells
- ELISA detection of DNA isolated from cells or tissues
- Immunofluorescence staining test.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you at the first time.
All services and products are for scientific use only, not for medical use！
