Although it is primarily thought of as a protein folding factory, the ER is the largest Ca²⁺ store in the cell. Abnormal calcium distribution within the endoplasmic reticulum usually implies a state of endoplasmic reticulum stress. Monitoring calcium distribution parameters in the ER can help to reasonably determine if and how they deviate during ER stress, which can then be used as an independent measure of ER health.

Fig. 1 Calcium and ROS crosstalk between endoplasmic reticulum and mitochondria (Görlach, Bertram, Hudecova & Krizanova, 2015)

As a leader in the field of cellular stress, Creative Bioarray can monitor the calcium distribution of the ER using several chemical and genetic tools. And based on the monitoring results, we can help our clients assess the status of ER and describe the causal relationship between disease pathogenesis and altered ER calcium stores.

ER Calcium Imaging Service - Chemical Calcium Ion Sensor

The probes we have selected have the following characteristics

• The ethoxy (AM) derivatives of these probes allow easy access of the dye to the cells.
• These markers have a very good signal/noise ratio and a large dynamic range.
• Easy to calibrate, some of them are ratiometric, i.e., they change excitation wavelength, emission wavelength, or both after binding to calcium, thus avoiding artifacts related to the geometry of the cell.

Workflow

• Selection of a suitable chemical class of calcium ion indicator.
• Treatment of the cells to be tested with the working solution.
• Record the dynamic changes in calcium ions and help our clients assess the status of ER based on the monitoring results and describe the causal relationship between the pathogenesis of the disease and the altered ER calcium stores.

ER Calcium Imaging Service - Genetically Encoded Calcium Indicators

Workflow

• Selection of suitable calcium ion indicators of the GECIs class.
• Introduction of calcium ion indicators into the target location by viral infection.
• Recording the dynamic changes of calcium ions and helping our clients to assess the status of ER based on the monitoring results, describing the causal relationship between the pathogenesis of the disease and the altered calcium stores of ER.

Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.

Reference

1. Görlach, A., Bertram, K., Hudecova, S., & Krizanova, O. (2015). Calcium and ROS: A mutual interplay. Redox Biology, 6, 260-271.

Services

• Cell Level Oxidative Stress Assay Services
  • DNA & RNA Oxidative Stress Detection
  • Lipid Peroxidation Stress Assay
    • Lipid Peroxidation By-products Assay
    • Low Density Lipoprotein Oxidative Stress Assay Service
    • High Density Lipoprotein Oxidative Stress Assay Service
  • Protein Oxidation and Nitration Assays
  • Reactive Oxygen Species Assays
    • Hydrogen Peroxide Assays
    • Nitric Oxide Assays
    • Intracellular ROS Assay
    • In Vitro ROS & RNS Assay
  • Cell Antioxidant Assay Services
    • Antioxidant Capacity Assays Services
    • Antioxidant Enzyme Activity Assays
  • Oxidase & Peroxidase Activity Assay Service
  • Stressed Cell Migration Assay
  • Oxidative Stress Induced Apoptosis Assay Services
  • Stressed Cell Viability and Proliferation Assay
  • Immunohistochemistry Detection Service of ROS
  • Blood OS State Assay Services
  • Urine OS State Assay Services
• Oxidative Stress Cell Model Establishment Services
  • Establishment of Stress Model by Chemical Method
    • Extracellular H₂O₂ Exposure Model System
  • Hyperoxia Cell Oxidative Model System
  • Quality Evaluation Index of Oxidative Stress Cell Model
  • Digestive System Stress Model
    • Intestinal Epithelial Oxidative Stress Cell Model Construction Service
      • Intestinal Epithelial Cell Modeling Methods
      • Intestinal Epithelial Cell for Oxidative Stress Modeling
    • Hepatic Stellate Cell Oxidative Stress Model
      • Rat Hepatic Stellate Cells Oxidative Stress Model
      • Human Hepatic Stellate Cell Oxidative Stress Model
    • Hepatoma Cell Oxidative Stress Model
    • Hepatocyte Oxidative Stress Model
      • Human Hepatocyte Stress Model
      • Hepatocyte Mitochondrial Stress Autophagy Model
      • Chicken Hepatocyte Oxidative Stress Model
  • Reproductive System Oxidative Stress Model
    • Rat Ovarian Oxidative Stress Model
    • Mouse Ovarian Oxidative Stress Model
    • Human Ovarian Granulose Cells Oxidative Stress Model
    • Male Reproductive Cell Oxidative Stress Model
  • Endoplasmic Reticulum Stress Model
    • Liver Cancer Cells ER Stress Model
    • Rhinovirus Vector for ER Stress Modeling
    • Osteoblast ER Stress Model
    • Hepatocyte ER Stress Model
    • Cardiomyocyte ER Stress Model
  • Establishment of Stress Model by Biological Method
    • Porcine Circovirus Type 2 Induction
  • Skeletal System Oxidative Stress Modeling Service
    • Xanthine-Xanthine Oxidase Modeling Service for Osteoblast
    • H₂O₂ Oxidase Modeling Service for Osteoblast
    • Fluoride Oxidase Modeling Service for Osteoblast
    • Chondrocyte Biological Oxidative Stress Modeling Service
    • Chondrocyte Chemical Oxidative Stress
    • Osteoblast Oxidative Stress Level Evaluating Service
    • Skeletal System Antioxidant Screening Service
    • Skeletal System Antioxidant Candidate Evaluating Service
  • Circulatory System Oxidative Stress Modeling Service
    • Myocardial Oxidative Stress
      • Establishment of Myocardial Oxidative Stress Model
      • Cardiomyocyte Antioxidant Evaluation Service
      • Medicine Cardiac Toxicity Assessment Service
      • Gene-Level Stress Assessment of Cardiomyocyte
    • Umbilical Vein Endothelial Cell Oxidative Stress Modeling Service
    • Umbilical Vein Endothelial Cell Antioxidant Evaluation Service
  • Nervous System Oxidative Stress Modeling Service
    • Neural Cell Oxidative Stress Modeling Service
    • Neuroblastoma Oxidative Stress Modeling Service
    • Glioblastoma Oxidative Stress Modeling Service
    • Oxidation Neural Cell Gene Expression Analysis Service
    • Nervous System Antioxidant Candidate Assessment Service
    • Neural Cell Oxidation State Regulation Service
  • Skin System Oxidative Stress Modeling Service
    • Skin Fibroblast Oxidative Stress Service
      • Efficacy Evaluation Service for Anti-aging Substances
      • Regulation of Stress State in Skin Fibroblasts
    • Melanocyte Oxidative Stress Service
  • Animal Stress Modeling and Evaluation Services
    • Animal Oxidative Stress Modeling and Evaluation Services
      • Chicken Embryo Oxidative Stress Modeling Service
      • Pig Oxidative Stress Modeling Service
    • Animal Immune Stress Modeling and Evaluation Services
• Cell Oxidative Stress Assay Service Selection Guide
• Organelle Level Stress Assay
  • Mitochondrial Stress Test
    • Mitochondrial Oxidative Stress State Analysis
    • Mitochondrial Toxicity Assay
      • Functional Mitochondrial Toxicity Assay
      • Mitochondrial Respiratory Complex Assay
      • Mitochondrial Biogenesis Assay
      • HCS Mitochondrial Toxicity Assay
    • ROS Localization Services in Mitochondria
  • Endoplasmic Reticulum Stress Test
    • Continuous Analysis of ER Stress
    • Unfolded Protein Response Assay
    • Rapid Detection and Quantification Service of ER Stress Model
    • ER Stress Induction Platform
    • ER Redox State Monitoring
    • ER Calcium Distribution Measurement
    • Chemical and Genetic Tools to Assess ER Stress
  • Lipid Membrane Stress Test
• Non-oxidative Cellular Stress Assay
  • Hypoxia Stress Molecular Test Services
  • Heat Stress Molecular Test Services