ER Redox State Monitoring
The endoplasmic reticulum (ER) plays a key role in the oxidative folding of nascent proteins, so perturbations to ER homeostasis may lead to protein misfolding and subsequent pathological processes. Monitoring ER redox parameters should help rationalize if and how they deviate during ER stress, which can then be used as an independent measure of ER health.
Fig. 1 Summary of the activation of the different signaling outputs during ER stress (Sicari, Delaunay‐Moisan, Combettes, Chevet & Igbaria, 2019)
Service Overview
Genetically encoded fluorescent protein probes are based on fluorescent proteins that have been mutated to redox-sensitive structures and reflect the degree of redox through fluorescence intensity measurements, indicating the redox status of the cell.
Creative Bioarray provides GSH and H2O2 monitoring of the endoplasmic reticulum through genetically encoded fluorescent protein probes and assesses the redox status of the ER accordingly.
ER Redox State Monitoring Service at Creative Bioarray
Available sensors
| Sensors |
Specificity |
Excitation wavelength |
Emission wavelength |
Region |
| roGFP |
GSH / GSSG |
395 / 465 nm |
505 nm |
Endoplasmic reticulum |
| HyPer |
H2O2 |
420 / 500 nm |
516 nm |
Endoplasmic reticulum, mitochondria, cytoplasm, peroxisome, nucleus |
Other sensors: ER-rxYFGP, Grx-rxYFP, PDI redox state, ERO1 redox state.
Monitoring methods
- Fluorescence
- FACS
- Microscopy
- Western blot
- Non reducing WB
Sensor Features
- Highly sensitive to changes in ROS content with a wide range of detection values.
- Specific for GSH and H2O2 molecules.
- Can be monitored dynamically in living cells.
- Localized to subcellular structures.
- More rapid than the cell's redox reaction.
- Not susceptible to interference by pH, cytochrome C, etc.
Service Applications
Explore fundamental questions in cell biology and free radical biology, including the molecular mechanisms that regulate the redox status of the cytoplasm, mitochondria, and endoplasmic reticulum.
Explore the role of ROS and endoplasmic reticulum redox signaling in diseases caused by various pathological factors.
Use in cultured cells to investigate the changes in H2O2 and its regulation in various physiological and pathological states, and also to detect H2O2 production in subcellular compartments such as the endoplasmic reticulum.
Applied to model organisms to observe the level of H2O2 at the level of the organism as a whole, realizing the advantages of real-time and visualization.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
Reference
- Sicari, D., Delaunay‐Moisan, A., Combettes, L., Chevet, E., & Igbaria, A. (2019). A guide to assessing endoplasmic reticulum homeostasis and stress in mammalian systems. The FEBS Journal, 287(1), 27-42.
All services and products are for scientific use only, not for medical use！
