Establishment of Stress Model by Chemical Method
Cell model is one of the effective methods to screen and evaluate antioxidants and study the mechanism of oxidative stress injury. Creative Bioarray provides many types of cellular stress services. Based on cell lines from nervous, urinary, immune, bone, circulation, digestion, skin and reproductive systems, we establish customized oxidative stress cell models for customers through chemical stressors.
There are many kinds of chemical stressors, among which hydrogen peroxide (H2O2) is the most common. As a kind of ROS, it can inhibit cell proliferation, cause oxidative damage of macromolecules in cells, and finally lead to serious consequences such as cell aging, death and mutation. We used H2O2 induced oxidative stress cell model. The model is generally used to explore the mechanism of free radical mediated cell injury and the protection and repair mechanism of antioxidants on oxidative damage.
PD Cell Model
Some chemicals with neurotoxicity can be applied to the study of neurological diseases. As the metabolite of neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), 1-methyl-4-phenylpyryl ion (MPP +) can inhibit mitochondrial complex I and cause excessive production of ROS, resulting in intracellular oxidative stress and cell apoptosis. We use this method to model PD cells for our customers.
Research Model of Blood Related Antioxidant Activity
Some chemicals can be used as free radical initiators, which can produce free radicals in free radical reactions, so they can also be used as stressors. 2,2-Azobis (2-methylpropylamidine) dihydrochloride (AAPH) is a small molecule azo initiator, which can induce the formation of peroxy free radicals and lead to various pathological changes through cell oxidative damage. The cellular stress model established with AAPH as the source of free radical production can be used to study the antioxidant activity of red blood cells, plasma and whole blood.
Model for Evaluating the Mechanism of Oxidative Stress in Cells and Tissues
Tert butyl hydrogen peroxide (t-BHP) is also a common free radical reaction initiator, which can be used as a co oxidant to evaluate the mechanism of oxidative stress in cells and tissues.
Other Induction Methods
In addition, drugs can also be used to build oxidative stress cell models. For example, doxorubicin (DOX) is an effective chemotherapeutic drug, but it can cause various cardiotoxicity such as tachycardia, arrhythmia and hypotension. Oxidative stress is a key factor for DOX to cause myocardial injury. This is because DOX can produce a large number of superoxide anion free radicals, resulting in mitochondrial dysfunction and cell injury.
There are many kinds of chemical stressors, including not only exogenous oxidants and neurotoxins, but also drugs with toxic effects on the body. They are widely used in the construction of oxidative stress cell model.
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All services and products are for scientific use only, not for medical use！
