Functional Mitochondrial Toxicity Assay
Figure 1. Schematic representation of the main mitochondrial functions in a cell. (Mihajlovic & Vinken, 2022)
Mitochondria are considered to be the powerhouse of the cell and play an important role in energy production, apoptosis, calcium signaling, and regulation of cellular metabolism. Also, mitochondria are common targets of drug-induced toxicity. Creative Bioarray offers a mitochondrial stress testing service. This service can be used to understand the mechanisms of cellular bioenergetics and mitochondrial toxicity. Creative Bioarray' functional mitochondrial toxicity assay service uses seahorse to detect the effects of compounds on oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) to assess cell metabolism and mitochondrial function.
This service can help our customers understand the specific mechanisms of cellular bioenergetics and mitochondrial toxicity.
Service Description
Our Functional Mitochondrial Toxicity Service provides insight into cellular bioenergetics and mitochondrial toxicity mechanisms by assessing mitochondrial stress. We use extracellular flux analyzers to assess mitochondrial function and cellular metabolism in real time.
We are committed to providing consistent, high-quality data with the flexibility to adapt protocols to meet specific client requirements.
In addition to mitochondrial toxicity, this service can be used for other applications to observe the transition between mitochondrial respiration and glycolysis in certain pathological conditions such as obesity, diabetes, cancer, cardiovascular disease, and neurodegenerative function.
Functional Mitochondrial Toxicity Assessment Protocol
| Media Assessed |
Unbuffered DMEM containing 10 mM glucose, 1 mM pyruvate and 2 mM glutamine |
| Cell Types Available |
H9c2, Huh7, HepG2, MCF-7, cropreserved human hepatocytes, cryopreserved rat hepatocytes (other custom cell lines available on request) |
| Test Article Concentration |
7 point dose response |
| Quality Controls |
Vehicle control
Positive control |
| Test Article Requirements |
50 µL of 50 mM DMSO solution or equivalent amount of solid compound. |
| Analysis Method |
Use of solid state fluorescent sensors to measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR). |
| Data Delivery |
Summary report
AC50 for OCR, reserve capacity and ECAR
Minimum effective concentration (MEC) for OCR, reserve capacity and ECAR |
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
Reference
- Mihajlovic, M., & Vinken, M. (2022). undefined. International Journal of Molecular Sciences, 23(6), 3315.
All services and products are for scientific use only, not for medical use！
