Glioblastoma Oxidative Stress Modeling Service
Glioblastoma, formerly known as glioblastoma multiforme (GBM), is one of the most aggressive cancers originating in the brain. Nerve cells strongly rely on aerobic metabolism to provide energy and are extremely sensitive to oxygen. Oxidative stress is an important cause of neuronal apoptosis. Excessive ROS in the body is related to the occurrence and development of Parkinson's disease, Alzheimer's disease, and other diseases, and is considered to be the cause of neuronal degeneration in patients.
Creative Bioarray can use Glioblastoma U373 cells and C6 cells as models to help our customers study the changes in signal transduction pathways, transcription factors, gas signal molecules, etc. After oxidative stress, and related pharmacological mechanisms of drugs.
U373 Cell Oxidative Stress Model
U373 is a human glioblastoma cell line. We used Fenton reagent or human neuromelanin to induce oxidative damage in this cell line.
C6 Cell Oxidative Stress Model
The glioma cell line C6 was cloned from rat glioma induced by N-nitrosourea by Benda et al. and was established after a series of in vitro cultures and animal passage.
Modeling method - H2O2
C6 cells in the logarithmic phase of growth were treated with H2O2 of different concentrations for 6 hours to determine the optimal induction concentration.
- MTT assay was used to determine the viability of cells under oxidative stress
- Determination of lipid peroxide and cell DNA cleavage rate to determine the degree of cell oxidative damage
- Determination of intracellular ROS content
- Lactate dehydrogenase assay
- Detection of apoptosis by in situ end labeling
- Expression of Bcl-2, Bax, caspase-3, and other apoptosis-related protein mRNAs in C6 cells
Model Applications
Effects of antioxidants on the injury of glioma cells
Study on gene transcription and protein expression of glioma cells under oxidative stress
To provide a basis for the study of Alzheimer's disease and other neurodegenerative diseases
To study the role of endotoxin in cell injury
To study the protective or toxic effects of melanin on nerve cells in Parkinson's disease.
Drug Management and Evaluation Services
We can also adjust the modeling process according to the specific needs of customers, provide drug administration experiments, pharmacology, efficacy evaluation, and pharmacokinetic analysis, and assist customers in studying the stress and/or pathological significance of the nervous system, which has important theoretical significance for identifying glioma cell-related diseases.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
