HCS Mitochondrial Toxicity Assay
Drug toxicity is usually a combination of multiple mechanisms. A single experimental method is unlikely to capture the complexity of cytotoxicity.
High content screening uses automatic fluorescence imaging to simultaneously analyze multi parameter indicators of cytotoxicity. It can detect the general cell death and / or cell death mechanism in the same cell population in the same well, and can cover a wide range of cellular pathological changes.
Creative Bioarray has the advanced high content screening equipment.
Creative Bioarray provides a cell-based cytotoxicity team to evaluate key toxicity markers, including cell number, nuclear concentration, total nuclear strength, cell permeability, mitochondrial membrane potential and cytochrome c release.
Service Description
The cells were incubated with many known toxic and nontoxic compounds at different concentrations. At the end of the incubation period, the cells were loaded with relevant dyes / antibodies and scanned with an automatic cell imager to determine a set of cell health markers.
Cytotoxicity panel using high content screening cell-based assay
| Analysis Method |
High Content Screening |
| Cell Types |
HepG2, U-87 MG, other custom cell lines |
| Toxicity Markers |
Cell loss
Nuclear size
Nuclear morphology
Cell membrane permeability
Mitochondrial membrane potential
Mitochondrial mass
Cytochrome c release |
| Cell Type |
HepG2 (others available on request) |
| Test Article Concentration |
8 point dose response curve up to 500 µM or solubility limit (different concentrations available) |
| Number of Replicates |
3 replicates per concentration |
| Quality Controls |
0.5% DMSO (vehicle control)
Chlorpromazine (positive control)
Valinomycin (positive control) |
| Data Delivery |
3-5 mg solid (depending on molecular weight) or equivalent DMSO solution |
| Data Delivery |
Minimun toxic concentration
Dose response curves |
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please Contact Us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
