Hepatocyte ER Stress Model
Endoplasmic reticulum is the main place for intracellular protein translation, synthesis, folding, modification and maturation. Various pathological conditions destroy the homeostasis of endoplasmic reticulum, and a large amount of unfolded and misfolded proteins accumulate, triggering endoplasmic reticulum stress (ERS). ERS plays an important role in the occurrence and development of many liver diseases. Creative Bioarray induced human normal hepatocyte line (L02 cells) with tunicamycin to construct human hepatocyte ERS model. This model is expected to provide an experimental basis for further exploring the molecular mechanism of ERS mediated hepatocyte apoptosis related diseases.
Technology Principle
Hepatocytes are rich in endoplasmic reticulum, which is one of the most sensitive cells of ERS. Tunicamycin is a commonly used inducer of ERS, which can inhibit the production of long terpenol tetradecose diphosphate in protein glycosylation pathway, make the sugar chain process hands and feet, form deglycoprotein, hinder the glycosylation modification of new proteins in endoplasmic reticulum, damage the function of endoplasmic reticulum, and finally cause ERS.
Through the gradient experimental model, we determined the optimal amount and treatment time of tunicamycin for the establishment of human liver cell L02 endoplasmic reticulum stress model.
After ERS, on the one hand, cells restore the function of endoplasmic reticulum through unfolded protein reaction (UPR).On the other hand, ERS is too strong or lasts too long, resulting in the balance and function of endoplasmic reticulum can not be reconstructed, and UPR reaction will induce apoptosis of damaged cells. Glucose regulatory protein 78 (GRP78) and CCAAT / hadron binding protein (CHOP) are marker proteins in ERS.
Service Content
We provide cell model construction and sales services
In addition, we can also adjust the modeling process according to the specific needs of customers, provide administration experiment, pharmacology, efficacy evaluation and pharmacokinetic analysis, and assist customers to study the physiological and / or pathological significance of hepatocyte ERS, which has important theoretical significance for clarifying digestive related diseases (hepatocyte apoptosis, etc.). We will determine the modeling situation by WB inspection of Er markers, detect the proliferation and survival of L02 by MTT method, and detect the apoptosis caused by tunicamycin by TUNEL method.
Model Application
Objective to study the ERS and signaling pathway of hepatocytes under the action of tunicamycin
To study the molecular mechanism of ERS mediated hepatocyte apoptosis related diseases
To investigate the physiological and / or pathological significance of hepatocyte ERS
To study the etiology of liver related diseases
The discovery and screening of antioxidants and the development of liver protective drugs
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Reference
- Koo, J., & Han, C. (2021). Signaling Nodes Associated with Endoplasmic Reticulum Stress during NAFLD Progression. Biomolecules, 11(2), 242.
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