Hepatocyte Mitochondrial Stress Autophagy Model
Nutritional deficiency, oxidative stress and metabolic stress can induce the increase of autophagy level. Oxidative stress is one of the common mechanisms of many stress injury factors. Mitochondria is an important organelle in cells and the target of many internal and external damage factors. Creative Bioarray established the autophagy model of human normal hepatocyte line L02 in vitro with oxidant tert-butyl hydroperoxide (t-BHP).
Modeling Application
Exogenous oxidative stress often plays a role by stimulating mitochondria to produce ROS through a variety of signal pathways, which is called "ROS induced ROS release".
- The model can be used to observe the role of mitochondrial oxidative stress and ROS related signaling pathway
- The model will provide an experimental means for the study of ROS directly induced autophagy
- The model can provide a reliable platform for further research on antioxidant regulation of autophagy targeting mitochondria
Modeling Principle
Human normal hepatocyte line L02 cells were routinely cultured in complete medium. After passage, L02 cells were treated with different concentrations of t-BHP to detect autophagy and ROS related indexes. We ensure the quality of modeling by means of cellular immunofluorescence staining, detection of cellular mitochondrial ROS level, and Western blot.
t-BHP Vs. H2O2
H2O2 is often used as an inducer of oxidative stress due to its long half-life. However, in practical experiments, H2O2 is still easy to decompose and is not easy to achieve more stable oxidative stress. One -(CH3) in the molecular structure of t-BHP replaces -OH in H2O2 and its stability is strengthened. After metabolism in vivo, H2O2 can be replaced and released again, so as to realize oxidative stress for a long time. Therefore, t-BHP has been used as an efficient and slow-release oxidant in many studies.
Services and Deliverable
We can also establish the oxidative stress autophagy model of other human hepatocyte cell lines according to the specific needs of customers, and find out the best induction dose. On the basis of establishing the model, we can provide intervention experimental services to evaluate the autophagy effect of some signal pathways in oxidative stress by adding specific targeted antioxidants or pathway specific inhibitors.
In addition, we also provide administration experiments, pharmacology, pharmacodynamic evaluation and pharmacokinetic analysis to help customers study the mechanism of liver injury and find drugs and antioxidants to prevent and treat liver injury.
Why Select Us?
- We used glucose oxidase to establish hepatocyte stress model instead of H2O2 directly
- At present, we have established the oxidative stress model of common human hepatocyte line L02, and provide customers with various testing services based on this model
- Our service is flexible. We can model the stress of other liver cell lines and provide relevant testing services for customers as required
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you at the first time.
All services and products are for scientific use only, not for medical use！
