High Density Lipoprotein Oxidative Stress Assay Service

High density lipoprotein (HDL) has antioxidant properties. It has been proved to be beneficial to cardiovascular disease. However, when HDL is oxidized (OxHDL), it has been shown to lose its cardioprotective properties and may actually be more dangerous than useful. The three main markers of OxHDL are MDA-HDL、CML-HDL and HNE-HDL, each of which can be found in the protein or lipid components of HDL. CML and HNE are generally more stable than MDA and may be a better choice when testing samples frozen for up to 6 months. MDA is best tested in fresh samples or samples frozen for up to one month.

Figure 1. High density lipoprotein oxidative stress (Miki, et al., 2019)

Creative Bioarray provides high density lipoprotein oxidation detection services. We detected and quantified OxHDL in plasma, serum or other biological fluid samples by enzyme immunoassay. The test results can be used to measure the level of cell stress.

HDL Oxidative Stress Assay Service At Creative Bioarray

Service Name	Application	Sensitivity
MDA-HDL ELISA Assay Service	Suitable for use with human plasma or serum samples	Detection sensitivity limit of <1 ng/mL for MDA-HDL
HNE-HDL ELISA Assay Service	Suitable for use with human plasma or serum samples	Detection sensitivity limit of 2 ng/mL for HNE-HDL
CML-HDL ELISA Assay Service	Suitable for use with human plasma or serum samples	Detection sensitivity limit of <1 ng/mL for CML-HDL

Sample Preparation Requirements

Serum and plasma: 240ul / sample.After quick freezing with liquid nitrogen, it shall be sealed and stored at - 80°C away from light and sent with dry ice.
Tissue: 200mg / sample.After quick freezing with liquid nitrogen, it shall be sealed and stored at - 80°C away from light and sent with dry ice.
Feces and intestinal contents: 200mg / sample, sub packed into 100mg / tube. After quick freezing with liquid nitrogen, it shall be sealed and stored at - 80°C away from light and sent with dry ice.
Cell: ≥ 1 × 10⁷/sample. After quick freezing with liquid nitrogen, it shall be sealed and stored at - 80°C away from light and sent with dry ice (one case shall be selected for every 20 samples and one more tube shall be sent for parallel control).

Note: all samples for lipid oxidation items are required to be sealed and stored away from light, and the words "lipid oxidation and light avoidance" are marked outside the test box. During sampling, the subjects should keep quiet (such as avoiding emotional excitement and sampling after exercise).

Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you at the first time.

Reference

Miki T, Miyoshi T, Kotani K, Kohno K, Asonuma H, Sakuragi S, Koyama Y, Nakamura K, Ito H. Decrease in oxidized high-density lipoprotein is associated with slowed progression of coronary artery calcification: Subanalysis of a prospective multicenter study. Atherosclerosis. 2019 Apr;283:1-6.

All services and products are for scientific use only, not for medical use！