Human Hepatocyte Stress Model
Oxidative stress is not only a part of liver dysfunction, but also the pathophysiological basis of liver injury. In order to carry out the experimental research related to hepatocyte oxidative stress, the experimental model of cell oxidative stress injury can be established by H2O2 in vitro.
However, in order to establish a stable hepatocyte oxidative stress model, Creative Bioarray uses glucose oxidase (GO) to simulate the oxidative environment. This method has the characteristics of stable H2O2. In order to improve the modeling quality, we observed the induction effect of different concentrations of GO on hepatocyte oxidative stress, and finally determined the appropriate concentration to establish hepatocyte oxidative stress model.
Modeling Principle
Oxidative stress is one of the important mechanisms of liver damage caused by liver diseases, including viral hepatitis, alcoholic liver disease and nonalcoholic fatty liver disease. GO and catalase form an oxidoreductase system, which can produce H2O2 at a stable concentration. Under the action of H2O2, the production of ROS increases. When it exceeds the scavenging capacity of the antioxidant system, ROS attacks adjacent tissues and cells, causing oxidative or reoxidation damage of DNA, protein and lipid, damaging the integrity of cell structure and function, causing apoptosis or necrosis, tissue damage, cancer and aging.
H2O2 Vs. Glucose Oxidase
In addition to its own oxidation, the oxidant H2O2 can also lead to OH -. It is often used to establish cell oxidative stress model, but H2O2 metabolizes rapidly in cell culture medium, the concentration is unstable, and the controllability of modeling effect is poor. GO and catalase form an oxidoreductase system, which can oxidize β-D-glucose generates hydrogen peroxide and D-gluconolactone. In this process, go is characterized by its ability to consume oxygen, catalyze glucose oxidation and produce H2O2 at a stable concentration.
Services and Deliverable
We can also establish the oxidative stress model of other human hepatocyte lines according to the specific needs of customers, and find out the best induction dose. Based on the established model, we provide administration experiment, pharmacopharmacology, pharmacodynamic evaluation and pharmacokinetic analysis to help customers study the mechanism of liver injury and seek drugs and antioxidants to prevent and treat liver injury.
Why Select Us?
- We used glucose oxidase to establish hepatocyte stress model instead of H2O2 directly
- At present, we have established the oxidative stress model of common human hepatocyte line L02, and provide customers with various testing services based on this model
- Our service is flexible. We can model the stress of other liver cell lines and provide relevant testing services for customers as required
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you at the first time.
All services and products are for scientific use only, not for medical use！
