Human Ovarian Granulose Cells Oxidative Stress Model
In recent years, studies have found that oxidative stress is closely related to many reproductive diseases. Ovarian oxidative stress not only affects oogenesis, follicular development, follicular maturation and follicular rupture, but also affects the quality of oocytes and embryos and early embryonic development, which will lead to the decline of female reproductive function. Therefore, it is of great significance to study the effects of oxidative stress on ovarian aging and reproductive function. The establishment of ovarian oxidative stress model is also of great value for studying the pathogenesis and screening new antioxidants.
Hydrogen peroxide (H2O2) has become an important tool for the preparation of cell oxidative damage model, which is widely used in various fields. Creative Bioarray uses human ovarian granulosa cells and H2O2 as inducer to construct an in vitro oxidative stress cell model, and evaluates the model through cell apoptosis and ROS level, in order to provide reference for the mechanism research and experiment of the effect of oxidative stress on ovarian and reproductive function.
Modeling Method
Under pathological conditions, granulosa cells in follicles will produce a large number of ROS, promote their own apoptosis and cause follicular atresia and degeneration, so as to reduce the quality of eggs and further reduce the fertilization rate and pregnancy rate. Therefore, in vitro ovarian granulosa cell oxidative stress model is a better method to evaluate follicular quality and ovarian function. H2O2 is a kind of reactive oxygen species with strong oxidation. It is an important link in the production of free radicals in the body. It is easy to obtain and relatively stable. The treated cells can produce a large amount of ROS in a short time, and then induce apoptosis. H2O2 is a reasonable inducer of oxidative stress of granulosa cells.
Service Content
Model purchase, feeding, modeling, material collection, and subsequent index detection. We can also provide model-based administration experiment, pharmacopharmacology, efficacy evaluation and pharmacokinetic analysis according to the specific needs of customers, so as to help customers study the mechanism of ovarian stress and seek new drugs for prevention and treatment.
We have explored the most suitable H2O2 concentration and the best treatment time for modeling. The model can be further applied to the experimental research related to ovarian oxidative stress, hoping to contribute to the research on the pathogenesis and treatment of ovarian function and reproductive aging related diseases.
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