In Vitro ROS & RNS Assay
Fig. 1 Physiological actions of ROS and RNS. (Chiurchiù et al., 2011)
Reactive oxygen species (ROS), such as superoxide and hydrogen peroxide, are continuously produced during cellular metabolism. Normally, the body uses antioxidant enzymes and other redox molecules to maintain the dynamic balance of ROS in the body. However, excess reactive oxygen species can lead to cellular damage, causing damage to DNA, lipids and proteins. Creative Bioarray provides two general detection methods of reactive oxygen species - intracellular ROS assay of intact cells and in vitro reactive oxygen species detection of cell lysates, tissue homogenates, blood or urine.
Technology Principles
We use probes coated with quenching reagents to detect total reactive oxygen species (ROS) and reactive nitrogen species (RNS) in various types of samples. This is a highly sensitive method where the probe will be rapidly oxidized to fluorescence detectable substances in the presence of ROS and RNS.
Scope of Application
Hydrogen peroxide, nitric oxide, peroxyl radical and persulfate anion detection.
Acceptable sample forms include urine, serum, plasma, cell lysate, or cell culture supernatant.
Service Process
Step 1. Prepare single cell suspension (tissue sample)
Step 2. Hemolysis (this step is not necessary for cell samples)
Step 3. Centrifuge to remove supernatant
Step 4. Adjust cell density
Step 5. Add the corresponding fluorescent probe
Step 6. Incubation
Step 7. Centrifuge the supernatant after washing
Step 8. On machine detection
Inspection and Delivery Standards
| Sample Type |
Requirement |
Transportation Conditions |
| Tissue |
The isolated tissues were made into single cell suspension and added into PBS medium |
Normal temperature transportation |
| Living cell |
Sample size: 1 X 106 cells / test, added into PBS medium |
Normal temperature transportation |
| Blood |
The blood was collected in the anticoagulant blood collection vessel of EDTA heparin sodium |
Normal temperature transportation |
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us or make online inquiry, and our customer service staff will help you at the first time.
Reference
- Chiurchiù, V., & Maccarrone, M. (2011). Chronic inflammatory disorders and their redox control: from molecular mechanisms to therapeutic opportunities. Antioxidants & redox signaling, 15(9), 2605–2641.
All services and products are for scientific use only, not for medical use！
