Intestinal Epithelial Cell for Oxidative Stress Modeling
Intestinal epithelial cells are vulnerable to oxidative damage. There are two methods to study intestinal epithelial cell damage: animal model and cell model. Using animal model alone has some disadvantages, such as high cost, instability, difficulty in mechanism research. It is very necessary to construct an appropriate cell culture model as a supplement to the animal model.
The modeling of intestinal epithelial cells must be inseparable from intestinal epithelial cells. According to the way of obtaining cells, cells cultured in vitro can usually be divided into primary culture cells and subculture cells. Creative Bioarray will select the most appropriate cell strain for model construction according to the actual research content of customers.
The following are the Intestinal Epithelial Cell we can provide for oxidative injury modeling at present. Each strain has its applicable simulation conditions.
Oxidative Stress Model of Primary Intestinal Epithelial Cells
If you choose to build a primary intestinal epithelial cell oxidative stress model, we will isolate, purify and identify the cells you selected. After obtaining the tissue block, we will carry out enzymatic separation and remove fibroblasts. The epithelial cell marker proteins were detected by immunohistochemistry and marker enzymes.
After obtaining the target cells, we will use physical or chemical stressors to induce oxidative stress damage according to your requirements.
The biggest advantage of primary culture is that the tissues and cells have just been isolated, and the biological properties have not changed greatly, which can better reflect the state in vivo to a certain extent. Especially in the confluence of cell culture, the expression of some special functions of primary culture is particularly strong. In such a culture stage, it can better show the morphological characteristics closely combined with the parent tissue.
Oxidative Stress Model of Passage Intestinal Epithelial Cells
At Creative Bioarray, we currently provide intestinal epithelial cell lines that can be subcultured for a long time, including but not limited to IEC-6 and IEC-18 Caco-2, HT-29, IPEC-J2, IPEC-1, etc. We mainly recommend customers to consider IEC-6, Caco-2 and IPEC-J2cell lines
IEC-6 cells
IEC-6 cells can rapidly proliferate and stably subculture when cultured in vitro, and have the characteristics of normal intestinal epithelial recess cells (with growth density inhibition, unable to form clones on soft agar, low adherence rate when inoculated at low density, a large number of microvilli on the cell surface and tight connections between cells). IEC-6 is widely used because it has no tumor gene. Please note: mouse derived cells are more suitable for basic theoretical research, which is different from traditional animal husbandry.
Caco-2 cells
Caco-2 cells are derived from human colon cancer cell line. Under normal cell culture conditions, cacb2 cells can differentiate spontaneously under certain culture conditions and form polar monolayer by connecting into pieces. These properties are similar to those of human intestinal absorptive cells.
IPEC-J2 cells
IPEC-J2 cells are derived from columnar epithelial cells of jejunum of piglets. As a small intestinal epithelial cell line without tumor gene, IPEC-J2 cell line has two main advantages as a conventional small intestinal epithelial cell oxidative stress model
- IPEC-J2 cells retain their own differentiation characteristics and have strong similarities with primitive intestinal epithelial cells. This makes it more suitable for the research of nutrition and animal diseases such as transmembrane transport, epithelial tight junction, flora adhesion.
- Compared with rodents such as IEC-6 and IEC-18, the digestive system of pigs is more similar to that of humans. Therefore, IPEC-J2 cells can not only be used in the study of nutrition and intestinal diseases of pigs, but also serve as a good model for the study of human intestine.
| Cell line |
Source |
Cancerous |
Passage number |
| IEC-6 |
Rat |
NO |
18-37 |
| IEC-18 |
Rat |
NO |
8-20 |
| Caco-2 |
Human |
NO |
22-55 |
| IPEC-J2 |
Swine |
NO |
50-80 |
| IPEC-1 |
Swine |
NO |
25-80 |
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you at the first time.
Reference
- Gonçalves P, Gregório I, Catarino TA, Martel F. The effect of oxidative stress upon the intestinal epithelial uptake of butyrate. Eur J Pharmacol. 2013 Jan 15;699(1-3):88-100.
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