Lipid Membrane Stress Test
Oxidative stress is thought to be associated with many pathological conditions, such as inflammation, atherosclerosis and neurodegenerative diseases. These reactions are thought to be a major cause of a variety of diseases, and oxidative stress caused by their reaction with lipids is thought to be closely associated with cardiovascular disease and cerebral ischemia. It seems important to assess lipid-related oxidative stress in order to understand the pathological conditions behind these diseases.
As leaders in the field of cellular stress, we use probes that can be localized to specific cellular regions and are useful for measuring regional oxidative stress, such as stress in cell membranes.
Creative Bioarray offers our customers a ROS localization service in lipid membranes. Through this service, we can specifically depict for our customers the different oxidative stress states of lipid membranes in response to specific exogenous oxidative stimuli, promising to reveal the detailed mechanisms of intracellular oxidative stress.
Principle of Lipid Membrane ROS Localization
Electron spin resonance (ESR) measurements are a useful method for the detection of free radical species in biological systems. Oxidative stress can be measured by ESR spectroscopy.
We use a derivative of TEMPO, a typical stable radical, as the detectable probe fraction of EPR. The probe is easily introduced into the cell, but also leaves the cell easily due to its amphiphilic nature. A TEMPO derivative localized to a specific cellular region can be used to measure oxidative stress, including stress on the cell membrane, and a TEMPO derivative localized to the lipid membrane would be advantageous.
The probe consists of 2 parts, a free radical part for ESR detection, and a fluorescent moiety for identifying its cellular distribution and a functional group for localization to the lipid membrane in the cell.
Our Facilities
- Confocal microscope
- EPR spectrometer equipped with a personal computer and data analysis application software
- Quartz flat-type EPR cuvette
- Conventional cell culture equipment
Lipid Membrane ROS Localization Service
Step 1: Preparation of Oxidative Stress Probes
Step 2: Cell culture and stimulation
Step 3: Loading the probe onto cells
Step 4: Confirmation of probe's cellular localization by confocal microscopy
Step 5: EPR measurement of lipid membrane oxidative stress probes
Step 6: Analysis and evaluation of oxidative stress data in target regions
Step 7: Pharmacological evaluation of oxidative stress (assessment of superoxide/hydrogen peroxide involvement, assessment of involvement of other ROS or ROS-related enzymes)
Service Applications
To elucidate the role of ROS in these different pathological conditions, it would be efficient to measure and assess oxidative stress at the subcellular level. This service can be used to specifically depict the different oxidative stress states of lipid membranes in response to specific exogenous oxidative stimuli, promising to reveal the detailed mechanisms of intracellular oxidative stress.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
