Low Density Lipoprotein Oxidative Stress Assay Service

Figure 1. Low density lipoprotein oxidative stress (Winklhofer-Roob, et al., 2017)

Oxidized low density lipoprotein (OxLDL) is a predictive biomarker of many diseases related to oxidative stress. LDL cholesterol is more dangerous when oxidized. Oxidized low density lipoprotein (OxLDL) is more reactive with surrounding tissues and can accumulate in the inner layer of the artery. Creative Bioarray provides low density lipoprotein oxidation detection service, and the detection results can be used to measure the level of cell stress.

The three main markers of OxLDL are MDA-LDL, CML-LDL and HNE-LDL, each of which can be found in the protein or lipid components of LDL. When detecting fresh samples, MDA LDL is the best choice because it is the most common modification in OxLDL. However, it is unstable in samples stored for more than one month. CML-LDL and HNE-LDL are more stable than MDA and may be a better choice when testing samples frozen for up to 6 months.

CML-LDL ELISA Service

Suitable for human plasma or serum samples.

The detection sensitivity limit of CML-LDL is 150 ng / ml.

HNE-LDL ELISA Service

Suitable for human plasma or serum samples.

The detection sensitivity limit of HNE-LDL is 150 ng / ml.

MDA-LDL ELISA Service

Suitable for human plasma or serum samples.

The detection sensitivity limit of MDA-LDL is < 50 ng / m

MDA is the most common modification found in oxidized LDL, but it is unstable in samples stored for more than one month. Our MDA-LDL assay is most suitable for fresh samples. For samples with long storage time, better results may be obtained by measuring CML or HNE modification, both of which are more stable than MDA.

Which Oxidized LDL ELISA Assay Service Should I Choose?

Each uses a different capture antibody for specific modification (CML, HNE, or MDA). MDA is the most abundant modification found in LDL, but it is also the most unstable. LDL is ideal for testing fresh samples. Samples stored at - 80°C for up to 6 months can be used to detect CML-LDL and / or HNE-LDL, which are more stable modifications.

Sample Preparation Requirements

Serum and plasma: 240ul / sample.After quick freezing with liquid nitrogen, it shall be sealed and stored at - 80°C away from light and sent with dry ice.
Tissue: 200mg / sample.After quick freezing with liquid nitrogen, it shall be sealed and stored at - 80°C away from light and sent with dry ice.
Feces and intestinal contents: 200mg / sample, sub packed into 100mg / tube. After quick freezing with liquid nitrogen, it shall be sealed and stored at - 80°C away from light and sent with dry ice.
Cell: ≥ 1 × 10⁷/sample. After quick freezing with liquid nitrogen, it shall be sealed and stored at - 80°C away from light and sent with dry ice (one case shall be selected for every 20 samples and one more tube shall be sent for parallel control).

Note: all samples for lipid oxidation items are required to be sealed and stored away from light, and the words "lipid oxidation and light avoidance" are marked outside the test box. During sampling, the subjects should keep quiet (such as avoiding emotional excitement and sampling after exercise).

Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you at the first time.

Reference

Winklhofer-Roob BM, Faustmann G, Roob JM. Low-density lipoprotein oxidation biomarkers in human health and disease and effects of bioactive compounds. Free Radic Biol Med. 2017 Oct;111:38-86.

All services and products are for scientific use only, not for medical use！