Male Reproductive Cell Oxidative Stress Model
Oxidative stress injury affects semen quality by affecting sperm concentration, reducing sperm motility, destroying sperm DNA integrity, changing sperm morphology and accelerating sperm apoptosis, and finally leads to male infertility. In recent years, oxidative stress injury of reproductive system has gradually attracted extensive attention. In order to clarify the mechanism of oxidative stress injury on reproductive system injury, it is very important to establish an oxidative stress model of male reproductive system.
Creative Bioarray established a male mouse reproductive system oxidative stress model for its customers by using a variety of oxidative stressors. After the establishment of the model, we took the conventional parameters of semen - semen concentration, sperm motility and sperm morphology as the basis, combined with biochemical tests, such as oxygen free radical, glutathione, malondialdehyde, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione-S-transferase (GST) Lactate dehydrogenase (LDH) and sperm DNA integrity were comprehensively evaluated.
Stressor Selection
Customers can choose stressors according to the needs of the actual test. The stressors we can provide include
| Classification of stressors |
Stressor |
Modeling method |
Principle |
| Heavy metal pollutants |
Nickel element |
Intraperitoneal injection |
It can form oxidative stress injury in medium and high dose groups |
| Cadmium element |
Intraperitoneal injection or gastrointestinal exposure to cadmium chloride |
Cadmium can lead to the decrease of glutathione level, the increase of lipid peroxidation, the change of intercellular stability, and the damage of DNA and cell membrane |
| Cigarette smoke exposure |
Nicotine, carbon monoxide, etc. |
Cigarette smoke exposure for 9 days can cause oxidative stress in semen |
These substances or their metabolites can appear in seminal plasma and cause reproductive toxicity |
| Organic industrial pollutants |
Tetrabromodiphenyl ether |
Intragastric administration of tetrabromodiphenyl ether in rats |
The degree of oxidative stress was positively correlated with the concentration of tetrabromodiphenyl ether |
| Deltamethrin |
Intraperitoneal injection |
It can increase the levels of nitric oxide and malondialdehyde in seminal plasma, damage the integrity of sperm DNA and increase cell apoptosis, and form oxidative stress to damage testicular tissue |
| Tetrachlorodibenzo dioxin |
Orally |
The enzyme like cytochrome P4501A1 is activated by binding and activating anti hyaluronidase in cytosol, resulting in semen oxidative stress |
| Ethylene glycol ethyl ether |
Orally |
|
| Lipopolysaccharide |
Lipopolysaccharide |
Intraperitoneal injection |
The immune reaction disrupts the blood testis barrier, increases leukocyte permeability, leads to the increase of leukocytes in semen, and then produces too much ROS and activates nitric oxide synthase |
| Hydroperoxide |
Tert butyl hydrogen peroxide (TBHP) |
Intraperitoneal injection |
It can be used as an inducer of oxidative stress in multiple systems in organisms |
| hydrogen peroxide |
Intraperitoneal injection |
|
| Endocrine hormone |
Ethinylestradiol |
Oral ethinylestradiol in rats |
|
| leptin |
intraperitoneal injection |
|
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you at the first time.
All services and products are for scientific use only, not for medical use！
