Vitiligo is a common depigmented skin disease. The pathogenesis includes autoimmune damage, melanocyte self-destruction and neurogenic theory. The synthesis of melanin by melanocytes produces quinone and phenolic intermediates, some of which are unstable and can form reactive oxygen species clusters (ROS), and these intermediates and ROS have toxic effects on melanocytes, called toxic melanin precursors.
Establishing a successful and reliable model of oxidative stress is important to partially reveal the pathogenesis of vitiligo as well as to study and screen antioxidant drugs. Hydrogen peroxide is an important reactive oxygen species that is highly permeable to cell membranes and forms highly reactive free radicals with intracellular iron ions through the Fenton reaction, leading to a series of reactions that are easily accessible and relatively stable in nature, so it has become an important tool for studying oxidative damage in cells.
Creative Bioarray can help our customers to model oxidative stress in melanocytes (immortalized melanocyte line PIG1) and melanoma cells (M14) through H2O2. Based on the above models, we can provide screening and evaluation services for antioxidant and anti-aging drug/component candidates. As an expert in the field of cellular stress, we can provide a one-stop service for our clients. Meanwhile, our experimental protocols are very flexible, and clients can choose different protocols according to their needs.
PIG1 has perfect melanin metabolism function and other biological properties, which are closer to human primary melanocytes, and thus has strong specificity; it can also overcome the instability of induction conditions caused by heterogeneity of primary melanocytes due to the age of the individual from which they are taken to the maximum extent, and thus has strong stability.
The modeling means are mild, and the damage caused by H2O2 does not cause cell death and may be repaired under the action of suitable antioxidants.
Stress modeling based on selected melanocytes or melanoma cells. We typically use hydrogen peroxide as a source of oxidative stress. Upon request, we provide anti-oxidant radical drug/antioxidant administration experiments, pharmacological pharmacology, efficacy evaluation, and pharmacokinetic analysis.
Customers provide established stress cell lines or oxidatively damaged tissues. After cell or tissue culture, we provide vitiligo drug/antioxidant administration assay, pharmacological pharmacology, efficacy evaluation and pharmacokinetic analysis.
We can evaluate the antioxidant capacity and ability to rescue oxidative damage of the drug candidate provided by the client and provide recommendations for efficacy evaluation and confirmation of the drug candidate.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.