Mitochondrial Biogenesis Assay
Creative Bioarray uses mitochondrial biogenesis assay to detect mitochondrial toxicity of new therapies. We can provide consistent, high-quality data, and flexibly adjust agreements according to specific customer requirements.
Service Description
There are some parallels between mitochondrial biosynthesis and bacterial and viral replication. Some drugs can affect mitochondrial biosynthesis by inhibiting the synthesis of mitochondrial-encoded proteins. Therefore it is recommended that all antiviral drugs be tested for their effects on mitochondrial function.
Creative Bioarray uses advanced screening and fluorescently labeled antibodies to assess the proportion of mitochondria-encoded proteins.
Mitochondrial Biogenesis Assay Protocol
| Method |
High content screening using fluorescently labelled antibodies for COX-1 and SDH-A |
| Cell Types |
Seahorse XFe96 flux analyser (Agilent Technologies) |
| Analysis Method |
HepG2 or HepaRG (others available on request) |
| Exposure Time |
5-8 days depending on cell line and customer needs |
| Number of Concentrations |
10 concentrations (n=3 wells per concentration) |
| Positive Control |
Chloramphenicol |
| Negative Control |
Lincomycin |
| Data Delivery |
COX-1 protein expression
SDH-A protein expression
COX-1/SDH-A protein expression ratio
Cell loss |
Our mitochondrial biogenesis assay uses high-intensity imaging to detect COX-1 (mtDNA encoded) and SDH-A (nDNA encoded) protein expression. Using the ratio of cox-1/SDH-A protein expression, the specific effects on mitochondrial biogenesis can be determined.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
