Mouse Ovarian Oxidative Stress Model
Ovary is the source of mammalian eggs and regulates the secretion of estrogen. Physiological levels of reactive oxygen species (ROS) regulate follicular formation, egg maturation and ovulation, but high concentrations of ROS will lead to oxidative stress, induce follicular atresia and granulosa cell apoptosis, accelerate egg aging and affect the fertility of female mammals. However, due to moral and ethical reasons, the molecular mechanism of ROS regulating follicular development, ovulation, fertilization, early embryonic development and follicular atresia in human body is not clear, so it is very important to establish a reliable mammalian ovarian oxidative stress model. Creative Bioarray now provides three stressors: hydrogen peroxide, 3-nitropropionic acid and sodium arsenite. The mouse ovarian stress model is established by intraperitoneal injection, which establishes a research platform for further studying the molecular mechanism of oxidative stress and ovarian injury and screening antioxidants.
Service Content
Model purchase, feeding, modeling, material collection, and subsequent index detection. We can also provide model-based administration experiment, pharmacopharmacology, efficacy evaluation and pharmacokinetic analysis according to the specific needs of customers, so as to help customers study the mechanism of ovarian stress and seek new drugs for prevention and treatment.
H2O2 Induction
Modeling method
The ovaries of anesthetized mice were burned with a certain volume and concentration of H2O2 under sterile conditions. After suture, the mouse ovarian oxidative damage model could be established. The damaged ovaries were taken, and the appearance and tissue sections were observed.
Features
The advantage of this model is that during the modeling time, the pathological structure is very obvious, which is easy to observe and obtain materials. It is more suitable for short-term oxidative stress experiments.
3-nitropropionic acid induction
3-NPA was injected intraperitoneally into healthy female rats not in estrus, once a day for 14 consecutive days. After sampling, the follicular development was examined, and the oxidative stress level and antioxidant enzyme activity (T-SOD, GSH PX) of ovarian tissue were detected.
Sodium arsenite induced
Modeling method
3-NPA is an inhibitor of mitochondrial complex II. It can inhibit the activity of mitochondrial succinate dehydrogenase, affect cell energy production, trigger the production and release of mitochondrial ROS, cause mitochondrial DNA damage and affect mitochondrial function. The mouse model of ovarian oxidative stress was simulated by intraperitoneal injection. The model can be used to study the effect of arsenic exposure on the reproductive ability of female rats. After modeling, we will detect the contents of ROS, MDA, SOD and GSH PX in ovarian tissue homogenate.
Features
This is a reliable mouse ovarian oxidative stress model. In this model, the level of ovarian oxidative stress increases and the abnormal distribution ratio of mitochondria in oocytes increases, which affects the follicular development and hormone metabolism balance, and affects the fertility of mice. The model is simple and easy to operate. It can be used to study the mechanism of oxidative stress on follicular development and to screen antioxidants that prevent ovarian oxidative stress.
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