Nervous System Antioxidant Candidate Assessment Service
The nervous system is one of the most important systems in the human body. The occurrence of neurodegenerative diseases is closely related to the increase of donor oxidation products. It will bring severe pain and burden to patients. The relationship between free radicals and diseases has become a research hot spot in the field of modern medicine and life sciences. Antioxidants can reduce or eliminate free radicals, prevent or reduce the damage of free radical oxidative stress. A large number of anti free radical drugs or antioxidants have been developed, and even free radicals have been used as auxiliary indicators for disease diagnosis.
Creative Bioarray can now provide screening and evaluation services for candidates of antioxidants and anti-free radical drugs based on the oxidative stress model of neuron cell / neuroblastoma cell line / glioblastoma cell line. We will develop specific antioxidative stress programs and endoplasmic screening systems to help our clients assess the level of oxidative stress and the level of cell death.
As an expert in the field of cell stress, we can provide customers with a one-stop service. At the same time, our experimental scheme is very flexible, and customers can choose different schemes according to their own needs.
Service Case
Ischemia-reperfusion injury can cause brain injury. Drug X is a candidate drug for the treatment of cerebral ischemia-reperfusion injury. We used H2O2 induced PC12 cell injury to simulate the in vitro model of cerebral ischemia-reperfusion injury, and explored the protective effect and mechanism of drug X.
Experiment design
PC12 cells in logarithmic growth phase were randomly divided into normal group, H2O2 treatment group and candidate drug X low, medium and high concentration groups. H2O2 group and candidate drug treatment group were induced with H2O2 for 24 hours in advance, and then candidate drug groups were administered with different concentrations respectively. H2O2 group and normal plants were cultured in the same volume of medium.
Analyzed parameters
- PC12 cells were cultured for 24 hours and the cell viability was detected by MTT assay
- After PC12 cells were cultured for 24 hours, the activities of Caspase-3 and caspase-9 were detected by colorimetry
- After PC12 cells were cultured for 6 hours, the intracellular ROS content was determined
- After PC12 cells were cultured for 6 hours, LDH release, MDA content, SOD, cat and GSH-PX activities were detected
- The expression of bax and bcl-2 protein in PC12 cells was detected by Western blot after cultured for 6 hours
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
