Neural Cell Oxidation State Regulation Service
Ischemic stroke is one of the diseases that seriously endanger human health. It has a high mortality and disability rate. Oxidative damage is one of its important pathogenesis. In order to explore its mechanism, it is sometimes necessary to study the signal pathway at the gene level. micro RNA is a kind of 18-25nt long, highly conserved non coding single stranded micro RNA. It regulates its expression by specifically recognizing mRNA and participates in the regulation of cell proliferation, differentiation, development, death, metabolism and other pathophysiological processes.
Creative Bioarray has developed a one-stop platform for regulating oxidative stress in nerve cells. We can regulate and improve the state of oxidative stress in cells through micro RNA. First, according to the requirements of customers, simulate the environment of cell oxidative stress, and then study the expression changes of target genes based on the cell model. This service aims to help customers explore the interaction between cellular oxidative stress and specific genes, and provide customers with the regulation scheme of neuronal oxidative stress state.
Service Case
Using the Neuro-2a oxidative damage model induced by hydrogen peroxide, the effects of microRNA fragment x pretreatment on cell survival rate, the protein expression of intracellular manganese superoxide dismutase (Mn-SOD) and extracellular superoxide dismutase (EC-SOD), the activities of total superoxide dismutase (T-SOD) and Mn-SOD, and the content of reduced nicotinamide adenine dinucleotide (NADH) were detected, To investigate the effect of micro RNA fragment x on oxidative stress injury in Neuro-2a cells.
Experimental design
Neuro-2a cells were cultured in DMEM medium containing 10% fetal bovine serum in a 5% CO2 incubator at 37 ℃. They were divided into 3 groups and treated differently
- Normal control group
- Hydrogen peroxide stimulated group
Hydrogen peroxide was added to the culture medium for 6 hours
- micro RNA fragment X pretreatment group
microRNA fragment X was transfected and stimulated with hydrogen peroxide for 6h after 48h.
Test parameters
- The cell survival rate was detected by CCK-8 method, the absorbance was detected by microplate reader, and the cell survival rate was calculated.
- Detection of T-SOD, Mn-SOD activity and NADH content.
- The expression of SOD was detected by Western blotting.
In conclusion, this study confirmed that micro RNA fragment x could reduce the death of Neuro-2a cells induced by hydrogen peroxide. Micro RNA fragment x plays a protective role in cell oxidative damage by enhancing the ability of neurons to scavenge reactive oxygen species, promoting cell energy metabolism, and enhancing the formation and function of synapses. This provides a new idea and basis for the use of mi-RNA in the prevention and treatment of ischemic stroke.
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All services and products are for scientific use only, not for medical use！
