Neural Cell Oxidative Stress Modeling Service
Various neuropathological studies have shown that the occurrence of neurodegenerative diseases is closely related to the increase in the level of oxidation products of donors. Because the brain and nerves contain a large number of lipids, the oxygen consumption is high, and neurons cannot divide, the damage caused by ROS accumulation may be an important inducement for the occurrence and development of diseases.
Creative Bioarray can use PC12 cells and N2a cells as models to help our customers study the changes in signal transduction pathways, transcription factors, gas signal molecules, etc. after oxidative stress, as well as the related pharmacological mechanisms of drugs.
Cell Line Introduction
Rat adrenal pheochromocytoma line (PC12) is a good model to study nerve cells' physiology, pathology, and pharmacology. The full name of N2a cells in mouse neuroblastoma is N2a cells, or Neuro-2a. It is a mouse neuroblastoma mother cell. At present, the cell line is mainly cultured in the laboratory as a cell model for in vitro pathological research, and it is also a common cell model for Alzheimer's disease research.
What We Do
Neuronal cell culture and modeling
Treatment of logarithmic growth stage neuronal cells (PC12, N2a or other neural cells) with different concentrations of H2O2 for a certain period of time induces cellular stress.
Our model quality evaluation system
- MTT assay was used to determine the viability of cells under oxidative stress
- The activities of Caspase-3 and caspase-9 were detected by colorimetry
- Determination of intracellular ROS content
- Detection of LDH release, MDA content, SOD, CAT activities
- The expression of Bax and BCL-2 protein was detected by Western blot
Antioxidant Rescue Evaluating Service in Neural Cell
Using established models of oxidative stress in neuronal cells, we can help our clients explore the antioxidant effects of antioxidants in cells and provide corresponding experimental reports. Observe the changes of intracellular lipid peroxidation products (MDA) and antioxidant enzymes (SOD, GPX) activities before and after the effect of antioxidants.
Model Application
- Common ischemia-reperfusion injury models in vitro
- The model can be used to explore the protective effect and mechanism of the target substance on oxidative damage of nerve cells
- Excessive ROS in the body is related to the occurrence and development of Parkinson's disease, Alzheimer's disease, and many other diseases, and is considered to be the cause of neuronal degeneration in patients.
- Neuronal cells are strongly dependent on aerobic metabolism for energy and are extremely sensitive to oxygen. This model is essential for the study of oxidative stress as a mechanism to induce neuronal apoptosis.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
