Neuroblastoma Oxidative Stress Modeling Service
Nerve cells strongly rely on aerobic metabolism for energy supply and are extremely sensitive to oxygen. Oxidative stress is an important cause of neuronal apoptosis. Excessive ROS in the body is related to the occurrence and development of Parkinson's disease, Alzheimer's disease, and many other diseases, and is considered to be the cause of neuronal degeneration in patients.
Creative Bioarray can use SH-SY5Y cells and SK-N-SH cells as models to help our customers study the changes in signal transduction pathways, transcription factors, gas signal molecules, etc. After oxidative stress, and related pharmacological mechanisms of drugs.
SK-N-SH Cell Oxidative Stress Model
SK-N-SH is a human neuroblastoma cell line, which was established by J.L.Bieder. It differs from SK-N-MC in that it has a long doubling time and dopamine- β - hydroxylase activity was high. SK-N-SH was used as a target cell line in a cell-mediated cytotoxicity test.
Modeling method
Using Fenton Reagent (FR) as a direct inducer of oxidative damage, SK-N-SH cells were exposed to oxidative stress.
SH-SY5Y Cell Oxidative Stress Model
SH-SY5Y cell is a subline of neuroblastoma SK-N-SH cell line after three clones. SH-SY5Y cells show moderate levels of dopamine- β - hydroxylase activity. The saturated density of SH-SY5Y cells was greater than 1 × 106/cm2.
Modeling method -H2O2
SH-SY5Y cells in the logarithmic phase of growth were treated with H2O2 of different concentrations for 24 hours to determine the optimal induction concentration.
- MTT assay was used to determine the viability of cells under oxidative stress
- Determination of lipid peroxide and cell DNA cleavage rate to determine the degree of cell oxidative damage
- Determination of intracellular ROS content
Modeling method CHP
Cumene hydroperoxide (CHP) is a stable lipophilic organic peroxide, which is widely used to induce intracellular lipid peroxidation and regulate gsh/GSSG levels. Foreign Studies on lipid peroxidation induced by organic peroxides mainly focus on erythrocytes, microsomes, and isolated hepatocytes, but there are no reports on the effects on nerve cells. Therefore, the study of oxidative stress induced by CHP on nerve cells has certain theoretical significance for the study of neurodegenerative diseases.
SH-SY5Y cells in the logarithmic growth phase were treated with CHP of different concentrations for 24 hours to determine the optimal induction concentration.
- Morphological observation and AO/EB staining
- Determination of cell inhibition rate and IC50 value
- Determination of intracellular ROS level
- Determination of intracellular MDA content
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All services and products are for scientific use only, not for medical use！
