Nitric Oxide Assays

Diabetes, renal ischemia, atherosclerosis, and inflammatory vascular diseases and cancer have been shown to be associated with an abnormal dynamic balance of nitric oxide in the body. Therefore, nitric oxide as well as nitric oxide synthase (NOS) can be used as one of the important indicators in the process of disease diagnosis and research.

Fig. 1 Generation of reactive oxygen and nitrogen species and antioxidant systems. (Costa et al., 2018)

Creative Bioarray provides two services for detecting nitric oxide in various samples.

Intracellular Nitric Oxide Analysis. A service that uses fluorescent probes to directly detect nitric oxide in intact cells in vivo, providing a wide range of pH stability from 5.5 to 9.0.

In Vitro Nitric Oxide Assay. Indirect measurement of nitric oxide by nitric oxide breakdown products (nitrite and nitrate) in cell lysates, tissue homogenates, plasma, serum, saliva, urine and cell culture supernatants.

Intracellular Nitric Oxide Assay

We use cell-permeable nitric oxide probes that enable rapid quantification of NO or NOS activity in cultured cells. The fluorescent probes we use are nitric oxide-specific and have good photostability and pH stability. The assay service is suitable for flow cytometry, fluorescence microscopy and fluorescent microplate assays.

In Vitro Nitric Oxide Assays

We use a simple colorimetric method to quantitatively determine no in various samples by NO₂^- / NO₃^-. First, nitrate (NO₃^-) in the sample is converted to nitrite (NO₂^-) by nitrate reductase. Next, total nitrite (absorbance 540 nm) as a colored azo dye product was detected. The kit is applicable to serum, plasma, urine, saliva, lysate and culture medium, and the detection sensitivity limit is about 2 µm (50 µl sample volume).

Service Process

Step 1. Prepare single cell suspension (tissue sample)
Step 2. Hemolysis (this step is not necessary for cell samples)
Step 3. Centrifuge to remove supernatant
Step 4. Adjust cell density
Step 5. Add the corresponding fluorescent probe
Step 6. Incubation
Step 7. Centrifuge the supernatant after washing
Step 8. On machine detection

Inspection and Delivery Standards

Sample Type	Requirement	Transportation Conditions
Tissue	The isolated tissues were made into single cell suspension and added into PBS medium	Normal temperature transportation
Living cell	Sample size: 1 X 10⁶ cells / test, added into PBS medium	Normal temperature transportation
Blood	The blood was collected in the anticoagulant blood collection vessel of EDTA heparin sodium	Normal temperature transportation

Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us or make online inquiry, and our customer service staff will help you at the first time.

Reference

Costa, N. T., Iriyoda, T., Alfieri, D. F., Simão, A., & Dichi, I. (2018). Influence of disease-modifying antirheumatic drugs on oxidative and nitrosative stress in patients with rheumatoid arthritis. Inflammopharmacology, 26(5), 1151–1164.

All services and products are for scientific use only, not for medical use！