Osteoblast ER Stress Model
Endoplasmic reticulum is an important organelle in cells. Its functional damage can cause endoplasmic reticulum stress (ERS). If persistent or too strong ERS cannot recover, it will induce apoptosis, resulting in various bone cell related diseases. Therefore, the role of ERS in bone biology has been paid more and more attention. The combined study of osteoblast ERS and osteogenesis is helpful to the study of bone biology under pathological conditions, and can provide a theoretical basis for the occurrence, diagnosis and treatment of bone diseases.
Creative Bioarray used tunicamycin, a recognized chemical that can cause ERS in osteoblast, as a stressor to model endoplasmic reticulum stress in human osteoblast.
Service Content
Osteoblast culture and modeling
Primary cultured human osteoblasts were established through standard process and identified by bone nodule and alkaline phosphatase. The cells were adherent cultured in the incubator. The culture medium was changed every 2 ~ 3 days according to the density of cell growth and the change of pH of the culture medium. The subculture was carried out when the confluence of cells reached 80%. A certain concentration of tunicamycin was used to intervene human osteoblasts for a certain time in order to induce endoplasmic reticulum stress.
Detection of subsequent indicators
We can use the osteogenesis PCR chip (including 84 genes related to osteogenesis such as alkaline phosphatase, osteocalcin, collagenase family, bone morphogenetic protein family and integrin family) technology to observe the osteogenesis expression of the tested osteoblasts.
Fluorescence quantitative PCR chip is a relatively simple and practical method to detect gene expression at present. It can detect the expression of 84 genes related to unfolded protein reaction at the mRNA level at one time. As a result, there is no need for real-time fluorescence quantitative detection. It has the advantages of high sensitivity, strong specificity, high amplification efficiency and good uniformity. It can study a large number of genes in the signal pathway at the same time and effectively avoid the blindness of single gene research.
Drug administration and evaluation services
In addition, we can also adjust the modeling process according to the specific needs of customers, and provide the model with administration experiment, pharmacopharmacology, efficacy evaluation and pharmacokinetic analysis, so as to assist customers in studying the physiological and / or pathological significance of osteoblast ERS, which is of great theoretical significance for clarifying orthopaedic related diseases (such as osteogenesis imperfecta and osteofluorosis).
Model Application
To study the ERS and osteogenesis signaling pathway of osteoblast under the action of tunicamycin
To explore the physiological and / or pathological significance of osteoblast ERS
To study the causes of orthopedic related diseases
Discovery and screening of antioxidants
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