Osteoblast Oxidative Stress Level Evaluating Service
Fig. 1 Effect of ROS and antioxidants on the activity of osteoclasts, osteoblasts and osteocytes in bone remodeling. (Domazetovic et al., 2017)
Creative Bioarray provides osteoblast oxidative stress level testing services.
Osteoblast apoptosis plays an important role in bone development and maintenance. To some extent, it leads to sex hormone deficiency, osteoporosis, glucocorticoid excess, and aging. Many studies have shown that oxidative stress caused by increased reactive oxygen species (ROS) can affect bone homeostasis. The development of new treatments requires understanding how ROS is involved in the regulation of bone biology, physiology, and pathology. The harmful effects of ROS in promoting oxidative stress and disease have attracted great attention in recent years. Among them, the relationship between oxidative stress and osteoporosis is the focus of research in recent years.
Service Content
The specimens confirmed as pathological bone tissue or the cell model of oxidative stress were placed in a sterilized Petri dish containing fetal bovine serum, and the adipose tissue and necrotic tissue were removed with tissue scissors. The samples were digested with trypsin and filtered to make cell suspension.
After the oxidative damage cell model is established, the pharmacodynamic indexes of the cells after oxidative damage must be detected. Through the comparison with the standard indexes, the feasibility of establishing the damage model can be determined or the conditions for further optimizing the establishment of the model can be used as a basis for measuring the pharmacological activity of the tested antioxidant active components and as a guide for the standardization of the oxidative damage cell model.
The most commonly used methods to detect whether osteoblasts are damaged by oxidative stress and the degree of damage mainly include the MTT colorimetric test to determine cell viability, electron spin resonance or chemiluminescence method to directly detect free radicals in oxidative damaged cells, thiobarbituric acid method (TBARS) to determine MDA, comet assay to detect DNA of oxidative damaged cells, and antioxidant enzymes including SOD The detection of GSH PX and catalase activity, the determination of total ROS level by dichlorofluorescein diacetate probe, and a series of other detection methods of apoptosis caused by oxidative stress.
Service Applications
This service can be used to effectively test that oxidative stress stimulation can cause cell death, help to clarify the role of oxidative stress in the pathogenesis of osteoporosis, and provide a certain theoretical basis for the development of new anti-osteoporosis drugs.
This service can also be used to evaluate the established oxidative stress model of human osteoblast-like cells. In addition, Creative Bioarray stimulates the oxidative stress model of human osteoblast-like cells using hydrogen peroxide, X / XO, and viruses. You can click the relevant links to learn more about our modeling services.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
Reference
- Domazetovic, V., Marcucci, G., Iantomasi, T., Brandi, M. L., & Vincenzini, M. T. (2017). Oxidative stress in bone remodeling: role of antioxidants. Clinical cases in mineral and bone metabolism: the official journal of the Italian Society of Osteoporosis, Mineral Metabolism, and Skeletal Diseases, 14(2), 209–216.
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