Oxidase & Peroxidase Activity Assay Service
Oxidases and peroxidases play a key role in the generation of reactive oxygen species during oxidative stress. Many oxidases and peroxidases use hydrogen peroxide as the primary substrate. Creative Bioarray offer a variety of assays to measure the activity levels of oxidase and peroxidase enzymes.
Monoamine Oxidase Assays
Fig. 1 Monoamine Oxidase Assays (Herraiz et al., 2018)
Our monoamine oxidase assay service uses Mao to react with a substrate to produce hydrogen peroxide, which is detected by colorimetric or fluorometric methods. The MAO activity in the unknown sample is calculated from the hydrogen peroxide standard curve.
Myeloperoxidase Activity Assays
Fig. 2 Monoamine Oxidase Assays (Stocker, Cassien, Vidal, Thétiot-Laurent & Pietri, 2017)
Myeloperoxidase (MPO) is a heme-based peroxidase enzyme that has been implicated in many disease states. In the presence of hydrogen peroxide, myeloperoxidase is converted to an active redox intermediary form (MPO-I). From there the enzyme plays two roles: a chlorination reaction by way of conversion of chloride ions to hypochlorous acid, and a peroxidation reaction where it is ultimately converted back to its native state.
Quantifies MPO chlorination activity from whole neutrophils, neutrophil lysates, tissue homogenates, or EDTA-plasma samples.
Peroxidase / Hydrogen Peroxide Assays
Our hydrogen peroxide / peroxidase detection service is a sensitive quantitative fluorescence detection of hydrogen peroxide or peroxidase activity level. In the presence of HRP, adhp reacts with H2O2 at a stoichiometric ratio of 1:1 to produce high fluorescent trihalogenate. We can detect peroxidase activity levels as low as 0.16 mu / ml and peroxide levels as low as 1 nm. This service is applicable to cell lysates, tissue homogenates, cell culture supernatants, plasma, serum, urine or other biological fluids. A simple analytical protocol can provide results in 30-90 minutes, depending on the type of sample.
Polyamine Oxidase Assay
Polyamine oxidase (PAO) is an enzyme that can oxidize various polyamines, including spermine and spermidine, to produce hydrogen peroxide. PAO is strongly induced by polyamine antitumor analogues, suggesting that PAO activity may be the cause of cell death through the production of hydrogen peroxide.
Our polyamine oxidase assay service measures PAO activity in biological samples. PAO reacts with the substrate to produce hydrogen peroxide. The signal generated by the reaction between hydrogen peroxide and the probe is proportional to the PAO level in the sample and can be detected in a fluorescence based microplate reader. PAO activity in unknown samples was calculated according to hydrogen peroxide standard curve.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you at the first time.
References
- Herraiz, T., Flores, A., & Fernández, L. (2018). Analysis of monoamine oxidase (MAO) enzymatic activity by high-performance liquid chromatography-diode array detection combined with an assay of oxidation with a peroxidase and its application to MAO inhibitors from foods and plants. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1073, 136–144.
- Stocker, P., Cassien, M., Vidal, N., Thétiot-Laurent, S., & Pietri, S. (2017). A fluorescent homogeneous assay for myeloperoxidase measurement in biological samples. A positive correlation between myeloperoxidase-generated HOCl level and oxidative status in STZ-diabetic rats. Talanta, 170, 119-127. doi: 10.1016/j.talanta.2017.03.102.
All services and products are for scientific use only, not for medical use！
