Oxidation Neural Cell Gene Expression Analysis Service
The relationship between free radicals and diseases has become a hot topic in the field of modern medicine and life sciences. Neuropathological studies have shown that the occurrence of neurodegenerative diseases is closely related to the increase of donor oxidation products. Because the brain and nerve contain a large amount of lipids, high oxygen consumption, and neurons cannot divide, the damage caused by ROS accumulation may be an important inducement for the occurrence and development of diseases.
A large number of anti free radical drugs or antioxidants have been developed, and even free radicals have been used as auxiliary indicators for disease diagnosis. Creative Bioarray can use neuron cell / neuroblastoma cell line / glioblastoma cell line as an oxidative stress model to help our customers study the changes of signal transduction pathways, transcription factors, gas signal molecules, etc. after oxidative stress, as well as the relevant pharmacological mechanisms of drugs.
In order to explore its mechanism, it is sometimes necessary to study some signal pathway proteins and protein expression related to cell stress at the gene level.
In order to explore the relationship between target genes and oxidative stress, Creative Bioarray has developed a one-stop experimental platform. First, according to the requirements of customers, simulate the environment of cell oxidative stress, and then study the expression changes of target genes based on the cell model. This service is designed to help customers explore the interaction between cellular oxidative stress and specific genes.
Service Case
Parkinson's disease (PD) is a common degenerative disease of the central nervous system. The main pathological change is the degeneration and loss of melanocytes, especially the dopaminergic neurons in the dense part of the substantia nigra. NF-kB is a nuclear transcription factor that widely exists in a variety of cells in the body. It participates in the signal transduction process of pathophysiology, gene regulation, immune response and apoptosis of a variety of diseases. NF-kB may protect nerve cells from oxidative damage. Our client wants to know whether chelate A can reduce oxidative stress damage.
Experimental scheme
Help customers to explore whether chelate A can reduce oxidative stress damage, use Fenton reagent to treat neuroblastoma cell line SK-N-SH, and establish a cell oxidative stress model. Confirm modeling quality through standard process. The expression of NF-kB mRNA was detected by real-time quantitative PCR, and the data were analyzed.
Service Content
We cultured neuroblastoma cell line SK-N-SH with standard procedure and established oxidative stress model with Fenton reagent.
- The cell survival rate was detected by CCK-8 method, the absorbance was detected by microplate reader, and the cell survival rate was calculated.
- Establish control group (FR) and treatment group (FR+ chelate A)
- The expression of NF-kB mRNA was detected by real-time quantitative PCR.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
