Protein Oxidation and Nitration Assays
In the presence of a variety of reactive oxygen species (ROS), cellular proteins will be affected by oxidative stress. Depending on the presence of ROS, damage to proteins may occur in the form of nitration or oxidation of various amino acid residues. ROS may also lead to the formation of advanced glycation end products (age) or advanced oxidative protein products (AOPP), both of which are stable markers of oxidative stress.
Fig. 1 Monoamine Oxidase (Umbarkar, P., 2015)
Creative Bioarray' protein carbonyl determination and protein nitration determination provide a rapid and sensitive method for the detection of carbonyl residues and 3-nitrotyrosine residues, respectively. Both are stable markers of oxidative stress and can be used in convenient Western blot formats and higher throughput ELISA.
Advanced glycation end products detection can be used to measure total age or specific age species as protein adducts. AOPP detection can provide very fast results in a standard microplate reader. Other detection methods of protein damage markers include our BPDE protein adduct ELISA, which can detect BPDE protein adducts quickly and reliably, and our S-glutathione adduct competitive ELISA, which can measure the s-glutathionation of proteins formed in the presence of ROS.
Our protein free radical detection service provides a unique method to measure protein free radicals produced by the presence of ROS and RNS. We use electron spin resonance (ESR) technology to capture free radicals into stable adducts that can be measured by ELISA.
Monoamine Oxidase Assays
Advanced Glycation End Products Assay Service
- AGE Assays
- CEL Assays
- CML Assay
- Methylglyoxal Assays
Advanced Oxidation Protein Products (AOPP) Assay Service
Measure AOPP formation in cell lysates, tissue homogenates, or plasma. Obtain results in less than 30 minutes.
Oxidized / Modified Human Lipoproteins Service
- Human LDL, Copper Oxidized
- Human LDL, MDA Modified
Protein Carbamylation Assays Service
- Anti-Carbamyl Lysine Antibodies
- Protein Carbamylation Sandwich ELISA
Protein Carbonyl Assays Service
- Protein Carbonyl ELISA
- Protein Carbonyl Fluorometric Assay
- Protein Carbonyl Immunoblot
- Protein Carbonyl-BSA
Protein Nitration Assays Service
- Anti-Nitrotyrosine Antibodies
- Nitrotyrosine ELISA
Protein Radical ELISA
Quantify protein radicals based on electron spin resonance (ESR) trapping and DMPO nitrone adduct formation. Suitable for use with cells, lysates, plasma or serum samples.
- Glutathione Protein Adduct ELISA
Quantify s-Glutathione protein adducts in a competitive ELISA format. Suitable for use with protein samples from cells, lysates, plasma or serum.
Why Select Us
- We have an experienced team of experts, ready to solve your problems
- Professional and high-quality services
- Products have passed strict inspections to ensure the accuracy of research
- Customized one-stop cost-effective technical services
- A variety of services related to cell stress, saving you time and effort
- We provide our customers with comprehensive support
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you at the first time.
Reference
- Umbarkar, P., Singh, S., Arkat, S., Bodhankar, S. L., Lohidasan, S., & Sitasawad, S. L. (2015). Monoamine oxidase-A is an important source of oxidative stress and promotes cardiac dysfunction, apoptosis, and fibrosis in diabetic cardiomyopathy. Free radical biology & medicine, 87, 263–273.
All services and products are for scientific use only, not for medical use！
