Regulation of Stress State in Skin Fibroblasts
Photoaging of skin refers to changes such as skin relaxation, increased and bulky wrinkles, skin thickening, roughness, pigmentation, and telangiectasia caused by long wave ultraviolet radiation. Skin photoaging is not only harmful to people's appearance, but also closely related to the occurrence of skin cancer. Therefore, more and more attention has been paid to elucidate the pathophysiological mechanism of photoaging and find more effective prevention and treatment measures.
miRNA is a kind of small non coding RNA with length of 20-22 nucleotides found in recent years. They can bind to the 3-terminal untranslated region of the mRNA of the target gene, causing mRNA degradation or blocking mRNA translation. It is well known that miRNA is involved in the regulation of cell proliferation, differentiation, development, metabolism, apoptosis and other physiological activities by regulating gene expression. Therefore, studying the role of miRNA in specific pathological processes can provide important ideas for the clinical prevention and treatment of the disease.
Creative Bioarray can help our clients to study the role of miRNA in the pathological process of skin photoaging. Help to study the regulatory role of miRNA in long wave ultraviolet (UVA) - induced photoaging model. As experts in the field of cell stress, we can provide customers with one-stop services. At the same time, our experimental scheme is very flexible. Customers can choose different schemes according to their own needs.
Service Process
Requirements
It is assumed that the expression of a protein during photoaging is regulated by miRNA. Therefore, it is necessary to detect the target miRNA and its expression in the UV induced photoaging model of skin fibroblasts, and to explore the relationship between them.
Optional modeling tools
- Acute stress of fibroblasts induced by UVB
- Fibroblast stress stimulated by moderate and low levels of ultraviolet radiation
- If you have other modeling requirements, please inform us in advance
Develop and exchange experimental programs
Human skin fibroblasts (HDFS) were isolated and cultured. The photoaging model was induced by different doses of UVA radiation. The expression of target miRNA and its target protein were detected by real-time quantitative PCR and western.blot, respectively.
HDFS were transfected with functional mimics of target miRNA and functional inhibitors of target miRNA respectively to detect the expression of target proteins. HDFS cells were transfected with target miRNA functional mimic, target miRNA negative control, target miRNA functional inhibitor and target miRNA control functional inhibitor respectively.
After 24 hours of transfection, they were given UVA radiation-induced photoaging model to detect the expression of target proteins.
Experiment execution
Data analysis and submission of experiment report
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