ROS Localization Services in Mitochondria
Electron transport in mitochondria can generate superoxide, so oxidative damage may accumulate more rapidly in mitochondria. Oxidative stress affecting mitochondria is thought to be closely associated with neurodegenerative diseases such as Alzheimer's and Parkinson's disease, and apoptosis.
As leaders in the field of cellular stress, we use probes that can be localized to specific cellular regions and are useful for measuring regional oxidative stress, such as that of mitochondria.
Creative Bioarray offers our clients a ROS localization service in mitochondria. Through this service, we can specifically depict for our customers the different oxidative stress states of mitochondria in response to specific exogenous oxidative stimuli, promising to reveal the detailed mechanisms of intracellular oxidative stress.
Mitochondrial ROS Localization Principle
Electron spin resonance (ESR) measurements are a useful method for the detection of free radical species in biological systems. Oxidative stress can be measured by ESR spectroscopy.
We use a derivative of TEMPO, a typical stable radical, as the detectable probe fraction of EPR. The probe is easily introduced into the cell, but also leaves the cell easily due to its amphiphilic nature.
The probe consists of 2 parts,
- One part is a lipophilic oxygen ion that can be localized to the mitochondria.
- The other part is a free radical moiety that can be used for ESR measurements.
Mitochondrial ROS Localization Service
Step 1: Preparation and Storage of Oxidative Stress Probes
Step 2: Cell culture and stimulation
Step 3: Loading the probe onto cells
Step 4: Confirmation of probe's cellular localization by confocal microscopy
Step 5: EPR measurement of intracellular oxidative stress probes
Step 6: Regional oxidative stress data analysis and evaluation
Step 7: Pharmacological evaluation of oxidative stress (assessment of superoxide/hydrogen peroxide involvement, assessment of involvement of other ROS or ROS-related enzymes)
Our Facilities
- Confocal microscope
- EPR spectrometer equipped with a personal computer and data analysis application software
- Quartz flat-type EPR cuvette
- Conventional cell culture equipment
Service Applications
To elucidate the role of ROS in these different pathological conditions, it would be efficient to measure and assess oxidative stress at the subcellular level. This service can be used to specifically depict the different oxidative stress states of mitochondria in response to specific exogenous oxidative stimuli, promising to reveal the detailed mechanisms of intracellular oxidative stress.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
