Skeletal System Antioxidant Candidate Evaluating Service
Bone is one of the most important tissues in the human body. Oxidative stress injury of chondrocytes and osteoblasts is closely related to arthritis and osteonecrosis, which will bring serious pain and burden to patients. The relationship between free radicals and diseases has become a research hotspot in the field of modern medicine and life sciences. Antioxidants can reduce or eliminate free radicals and prevent or reduce the damage of free radical oxidative stress. A large number of anti-free radical drugs or antioxidants have been developed one after another, and even free radicals have been used as auxiliary indicators for disease diagnosis.
Creative Bioarray can now provide screening and evaluation services for candidates of antioxidants and anti-free radical drugs based on the oxidative stress model of osteoblasts and chondrocytes. We will develop specific antioxidative stress programs and endoplasmic screening systems to help our clients assess the level of oxidative stress and the level of cell death.
As an expert in the field of cell stress, we can provide customers with a one-stop service. At the same time, our experimental scheme is very flexible, and customers can choose different schemes according to their own needs.
Service Cases
Experimental purpose
Evaluation of the protective effect of the candidate drug X on osteoblast injury
Experiment design
Calvarial osteoblasts of SD suckling rats were isolated and cultured. The experiment was divided into
- A blank group
- Model group
- X candidate drug treatment group
The oxidative stress injury model of osteoblasts was established with H2O2 while osteoblasts were injured by oxidative stress, osteoblasts were cultured with serum-containing X candidate drugs, and the blank group was not treated.
Analyzed parameters
The contents of malondialdehyde and superoxide dismutase in bone cells were measured
The expressions of nuclear factor E2 related factor 2 and heme oxygenase 1 protein in osteoblasts were detected by Western blot.
Results and conclusions
- Compared with the blank group, the content of malondialdehyde in the model group increased significantly and the content of superoxide dismutase decreased significantly.
- Compared with the model group, the content of malondialdehyde in the X candidate drug treatment group was significantly reduced and the content of superoxide dismutase was significantly increased.
- Compared with the blank group, the expressions of heme oxygenase 1 and nuclear factor E2 related to factor 2 in the model group were significantly increased.
- The expressions of heme oxygenase 1 and nuclear factor E2 related factor 2 in bone cells treated with X candidate drugs were significantly higher than those in the model group.
- The results show that X candidate drugs have a protective effect on the oxidative damage of osteoblasts.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you the first time.
All services and products are for scientific use only, not for medical use！
