Cells under oxidative stress will have abnormal migration, so the detection of cell migration can be used to evaluate the level of cell stress. Cell migration is one of the basic life activities of cells and an important indicator of cell characteristics. Cell migration is closely related to immune response, tissue repair, embryonic development, angiogenesis, metabolism and tumor metastasis. Its mechanism is related to the state of cells and environment (growth factors, chemokines, etc.). Creative Bioarray provides cell migration and invasion detection services for all our customers.
The most widely accepted cell migration technique is the Boyden Chamber assay. The classic transwell migration assay system uses a hollow plastic chamber, sealed at one end with a porous membrane. This chamber is suspended over a larger well which may contain medium and/or chemoattractants. Cells are placed inside the Chamber and allowed to migrate through the pores, to the other side of the membrane. Migratory cells are then stained and counted.
The wound healing assay simulates cell migration during wound healing in vivo, and it is a simple and inexpensive way to study directed cell migration in vitro.
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