Stressed Cell Viability and Proliferation Assay
Tests that measure cell proliferation, cell viability and cytotoxicity are often used to monitor the response and health of cells in cultures treated with various stimuli. The correct choice of detection method depends on the number and type of cells used and the expected results. The detection of cell proliferation can monitor the number of cells, the number of cell divisions, metabolic activity or DNA synthesis in a period of time.
The cells will be in a certain degree of oxidative stress and abnormal proliferation. Creative Bioarray provides detection services for oxidative stress cell viability and proliferation, so as to facilitate the evaluation and monitoring of cell oxidative stress level.
DNA Synthesis and Proliferation Detection Service
- BrdU cell proliferation assay
Cell proliferation can be studied by monitoring the incorporation of radioisotope [3H] - thymidine into cell DNA and autoradiography. Alternatively, 5-bromo-2'- deoxyuridine (BrdU test) can be used instead of thymidine. Cells doped with BrdU in DNA can be easily detected by using monoclonal antibodies against BrdU and secondary antibodies coupled with enzyme or fluorescence.
Metabolic Proliferation Detection Service
- MTT cell proliferation assay
MTT (3 - [4,5-dimethylthiazole-2-yl] - 2,5-diphenyltetrazole bromide; thiazole blue) is a water-soluble tetrazole salt, which will produce a light yellow solution when prepared in the medium or salt solution lacking phenol red. Dissolved MTT will be converted into insoluble purple methionine due to the cleavage of tetrazole ring by dehydrogenase. The water-insoluble methyl alcohol can be dissolved with isopropanol or other solvents, and the dissolved substances can be measured by spectrophotometry using absorbance as a function of the concentration of conversion dye.
- XTT cell proliferation assay
In contrast to MTT, the cutting product of XTT is soluble in water; Therefore, no dissolution step is required. Tetrazolium salt XTT is cleaved into methyl methacrylate through a complex cellular mechanism. This bioreduction occurs only in living cells and is associated with NAD (P) H production by glycolysis. The amount of nail dye formed is directly related to the number of metabolically active cells in the culture.
Luminous Cell Viability Testing Service
Since ATP is an indicator of metabolically active cells, the number of living cells can be evaluated according to the amount of ATP available. Luciferase detection of ATP cell viability can provide a highly sensitive homogeneous detection for quantifying ATP in cell culture. The kit uses firefly luciferase to oxidize D-luciferin, and evaluates the amount of ATP available in cell culture through the generated light. This sensitive detection method only needs to add the ATP detection mixture directly to the cells cultured in serum containing medium. No cell washing, medium removal or multi-step pipetting is required. The kit is sensitive enough to detect single cells or 0.01 pmol of ATP. The generated signal has linearity of six orders of magnitude. By correlating the amount of ATP with the number of living cells, the test has a wide range of applications, from determining the number of living cells to cell proliferation to cytotoxicity.
Creative Bioarray is dedicated to providing high-quality products, comprehensive services, and tailored solutions to support and facilitate life sciences and pharmaceutical research and development. If you have any questions or needs, please contact us, and our customer service staff will help you at the first time.
All services and products are for scientific use only, not for medical use！
